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在现场以单细胞分辨率的同位素追踪,使用质谱成像

Rosalie G J Rietjens^1,2,3, Bram Heijs^4,5

¹Department of Internal Medicine (Nephrology), Leiden University Medical Center, Leiden, The Netherlands. r.g.j.rietjens@lumc.nl.

Methods in molecular biology (Clifton, N.J.)

|October 1, 2024

概括

本研究引入了一种单细胞水平的动态代谢学方法,使用in situ稳定同位素跟踪和质谱成像 (MSI). 这种技术提供了空间代谢洞察力,以更深入地了解细胞过程.

科学领域:

生物化学生物化学
分析化学分析化学
细胞生物学细胞生物学

背景情况:

质谱成像 (MSI) 能够对组织进行无标签的空间分子分析.
最近的进展已经在MSI中实现了单细胞分辨率,补充了其他单细胞的OMIC技术.
代谢是动态的,理解代谢周转对于生物学见解至关重要.

研究的目的:

描述in situ稳定同位素跟踪的设置,其次是MSI支持的空间代谢学.
在单细胞水平上实现动态代谢.

主要方法:

在组织内在现场稳定同位素的追踪.
质谱成像 (MSI) 用于空间代谢学.
在单个单元格层面获取和分析数据.

主要成果:

对动态单细胞代谢学协议的演示.
组织的高分辨率空间代谢概况.
稳定同位素跟踪与MSI的整合.

结论:

描述的方法允许在单细胞水平上进行动态代谢分析.
这种方法增强了在空间背景下对细胞新陈代谢的理解.
MSI是先进的空间代谢学研究的强大工具.

关键词:

同位素追踪是一种同位素追踪.质谱仪成像成像质谱仪成像组织代谢基因组.振动组的切割振动组的切割

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