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口腔抹布作为直接牛微生物组采样的代理在荷尔斯坦乳牛的样本颜色与样本颜色相关
Joseph H Skarlupka1,2, Madison S Cox1,3, Andrew J Steinberger1,2
1Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, WI, United States.
Frontiers in microbiology
|October 2, 2024
概括
来自奶牛的较深的口腔抹布更好地代表了细菌的小肠微生物群,而较浅的抹布显示了更多的变化. 片颜色影响真菌社区的代表性,突出其对微生物组研究的重要性.
科学领域:
- 鲁门微生物组研究
- 乳牛的健康状况 乳牛的健康状况
- 微生物生态学 微生物生态学
背景情况:
- 口腔抹布可以捕获相当一部分奶牛牛皮的细菌群体.
- 口腔拭片的样品质量有所不同,可能会影响微生物组的表现.
- 口腔抹布特征,如颜色,对微生物组准确性的影响尚未得到充分理解.
研究的目的:
- 调查口腔抹布颜色与代表体细菌和真菌群体的准确性之间的关系.
- 为了确定更深色的口腔抹布是否提供了更具代表性的肠微生物组样本.
- 评估口腔抹布对于基于人群的微生物组研究的有用性.
主要方法:
- 收集了402头奶牛的口腔抽样和13头奶牛的乳头样本.
- 对细菌群体进行了16S rRNA测序,对真菌群体进行了ITS测序.
- 开发了一种新的成像方法,以色度计量扫描片的黑暗.
主要成果:
- 增加的扫描暗度得分与显著更高的细菌α多样性相关.
- 较轻的扫描显示了更大的社区结构变化,有更多的口腔/环境种群.
- 较深色的拭子与真菌α多样性降低有关,并且代表了体固体中的真菌群体.
结论:
- 口腔抹布颜色是影响细菌和真菌社区的代表性的关键因素.
- 较深色的拭子更多地表明了细菌肠微生物群,而拭子颜色影响了真菌社区的捕获.
- 这项研究完善了口腔抹布的使用,作为牛奶奶牛的体微生物组分析的替代品.
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