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使用LC-MS/MS测定血和全血中的ivermectin

Natpapat Kaewkhao¹, Warunee Hanpithakpong¹, Joel Tarning^1,2

¹Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.

Wellcome open research

|October 2, 2024

概括

一种新的,敏感的方法可以准确量化血液和血中的ivermectin. 这种经过验证的技术适合临床使用,有助于消除疟疾的努力和对虫病治疗的监测.

科学领域:

药理学药理学是指药理学的学科.
分析化学分析化学
寄生虫学的寄生虫学

背景情况:

在全球范围内,对治疗虫病和 filariasis 具有至关重要的意义.
由于其杀蚊活性,它显示出消除疟疾的潜力.

研究的目的:

开发和验证一种高吞吐量,敏感的方法来定量ivermectin.
使用自动抽样和LC-MS/MS进行血和全血分析.

主要方法:

采用96井板混合固相提取用于脂去除.
使用了Agilent Poroshell 120 EC-C18柱,具有特定的移动相条件.
在正电离模式下使用三重四极质谱仪进行检测.

主要成果:

在血和全血中的度为0.970384 ng/ml的方法得到了验证.
证明了高精度 (批量内和批量间<15%) 并没有检测到转载或矩阵效应.
在稳定性测试中证实了ivermectin的稳定性,没有观察到稳定性测试中的降解.

结论:

经过验证的LC-MS/MS方法具有高度灵敏,精确和选择性.
该方法适用于临床应用和对ivermectin的实验室量化.
这支持ivermectin在疾病治疗和预防策略中的作用.

关键词:

发展发展发展发展发展人类血液的人类血液.我从来没有使用过mectin.这是LC-MS/MS.疟疾:疟疾是一种疾病.验证验证验证验证

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