在Murrah水牛中检测多态性和表征精子细胞的染色质重塑相关基因
Harsimran Kaur1,2, Meenakshi Chitkara1,2, Eldho Mathai1,2
1ICAR- National Bureau of Animal Genetic Resources, Karnal (Haryana), India.
Tropical animal health and production
|October 2, 2024
概括
季节性变化会影响水牛牛的生育能力. 关键的精子基因原胺 (PRMs) 和过渡核蛋白 (TNPs) 保持高度,发现的遗传变异有限,这表明其他因素影响季节性精子质量.
科学领域:
- 动物繁殖 动物繁殖
- 遗传学 是一个遗传学.
- 精液生物学 精液生物学
背景情况:
- 季节性变化,特别是夏季的炎热,会对水牛的精液质量和生育能力产生负面影响.
- 了解影响这些季节变化的遗传因素对于有效的管理和改进策略至关重要.
- 染色体重塑基因,包括原胺 (PRMs) 和过渡核蛋白 (TNPs),在精子的发育和功能中起着至关重要的作用.
研究的目的:
- 在Murrah水牛公牛中鉴定PRM1,PRM2,TNP1和TNP2基因中的遗传多态性.
- 为了研究水牛公牛精液质量的季节性变化的遗传基础.
- 为提供关于水牛这些关键精子相关基因的遗传构成的全面报告.
主要方法:
- 测序PRM1,PRM2,TNP1和TNP2基因的编码和内基区域.
- 基因序列与牛同类基因序列的比较分析.
- 分析夏季和冬季之间的基因表达水平 (折叠变化).
主要成果:
- 在PRM1,PRM2,TNP1和TNP2中没有发现编码区域变异,这表明保护程度很高.
- 确定了两个内基变异 (PRM1中的G16C和PRM2中的G96A).
- 布巴林PRM1和TNP2基因序列与牛基因序列显著相同和相似.
- 在季节之间没有观察到PRM1,PRM2,TNP1和TNP2的基因表达水平的显著差异.
结论:
- 编码原胺蛋白 (PRM1,PRM2) 和过渡核蛋白 (TNP1,TNP2) 的基因在Bubalus bubalis中高度保留.
- 有限的遗传变异表明,除了这些特定基因中的编码区域多态化之外,其他因素也会导致水牛的季节性精液质量下降.
- 需要进一步的研究来探索其他影响水牛季节性生殖效率的遗传和环境因素.
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