关于培养来自患者的宫癌器官的协议
Rui Wang1, Timothy Harris1, Dalissa Negrón-Figueroa1
1Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
STAR protocols
|October 2, 2024
概括
这项研究详细介绍了从宫癌组织中建立患者衍生器官 (PDO) 的协议. 这些有机物有助于了解宫癌生物学和开发新疗法.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 生物技术是生物技术.
背景情况:
- 宫癌研究从患者衍生器官 (PDO) 中获益,用于模拟疾病.
- 从各种样本类型中获得可靠的PDO培养需要标准化的协议.
研究的目的:
- 为培养子宫癌原标产品提出一个全面的协议.
- 包括瘤活检/切除和细胞刷样本细胞的工作流程.
主要方法:
- 该协议涵盖PDO培养的启动,包括组织加工,解离和细胞分离.
- 它详细介绍了手术和细胞刷样本中的播种细胞.
- 提供有关PDO维护,通道和调节媒介生产的指导.
主要成果:
- 该协议使得成功建立和维护宫癌PDOs.
- 它证明了不同样本来源 (活检,切除,细胞刷) 的适应性.
结论:
- 该协议为产生和维持子宫癌PDO提供了一个有价值的框架.
- 它支持子宫癌的临床前研究和治疗开发.
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