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Updated: Jul 30, 2026

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
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使用ONE-GO生物传感器检测原始细胞培养中的内源GPCR活性的协议

Remi Janicot1, Mikel Garcia-Marcos2

  • 1Department of Biochemistry & Cell Biology, Chobanian & Avedisian School of Medicine, Boston University, Boston, MA 02118, USA.

STAR protocols
|October 2, 2024
PubMed
概括

这项研究引入了ONE向量G蛋白光学 (ONE-GO) 生物传感器,用于测量初级细胞中的G蛋白结合受体 (GPCR) 活性. 这些多功能生物传感器可以检测所有四大G蛋白家族,从而实现广泛的实验应用.

关键词:
生物技术和生物工程细胞生物学 细胞生物学细胞培养培养的细胞培养基于细胞的测试分子生物学分子生物学信号传输 信号传输

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相关概念视频

G-protein Coupled Receptors01:21

G-protein Coupled Receptors

G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
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GPCR Desensitization

G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...