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Updated: Jun 11, 2025

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
09:37

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

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扩大本地结构生物学工具箱:F 动态核极化与快速魔法角度旋转

Kumar Tekwani Movellan1,2, Wenkai Zhu2,3, Daniel Banks4

  • 1Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.

Science advances
|October 2, 2024
PubMed
概括

这项研究引入了-19动态核极化 (DNP) 与快速魔法角旋转 (MAS) NMR,以分析人体细胞内的蛋白质. 这种技术显著加快了数据采集的速度,使得对细胞蛋白质的详细结构和动态见解成为可能.

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Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
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Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling.  This phenomenon, called the Nuclear Overhauser Enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring...
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Many organic, inorganic, and biological molecules contain spin-half nuclei such as nitrogen-15, fluorine-19, and phosphorus-31. As a result, NMR studies of these nuclei have found extensive applications in chemical and biological research.
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