由葡萄糖度控制的微凝中酶触发的药物释放
Klaudia Kaniewska1,2, Marcin Mackiewicz2, Oleh Smutok3
1Faculty of Chemistry, University of Warsaw, 1 Pasteura, Warsaw, PL 02-093, Poland.
ACS biomaterials science & engineering
|October 2, 2024
概括
这项研究开发了智能微凝,可根据葡萄糖水平释放药物. 这些用葡萄糖氧化酶 (GOx) 修改的聚烯酸微凝具有针对性药物输送的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 药物输送系统 药物输送系统
- 纳米技术纳米技术
背景情况:
- 智能药物输送系统对于现代医学至关重要.
- 需要控制释放机制来提高治疗疗效.
- 酶反应性材料提供了新的药物输送策略.
研究的目的:
- 为控制药物释放设计和表征葡萄糖反应微凝.
- 研究葡萄糖度和缓冲条件对药物释放动态的影响.
- 评估这些微凝在利用增强透性和保留效应 (EPR) 的目标传递方面的潜力.
主要方法:
- 用葡萄糖氧化酶 (GOx) 修改的聚烯酸微凝的合成和表征.
- 传输电子显微镜 (TEM) 和动态光散射 (DLS) 用于尺寸分析.
- 在不同的葡萄糖和缓冲度下使用多克索鲁比辛 (DOX) 的体外药物释放研究.
主要成果:
- 合成的p ((AA-BIS) -GOx微凝,平均尺寸为130nm.
- 观察到由葡萄糖触发的微凝大小变化和显著的多克索鲁比辛释放,取决于葡萄糖和缓冲度.
- 在特定的缓冲条件下,在高葡萄糖度 (25mM与5mM) 下,在模仿瘤微环境的情况下,证明药物释放率更高.
结论:
- 开发的GOx修饰的微凝表现出受控的,酶触发的药物释放.
- 释放配置文件可以根据葡萄糖和缓冲度进行调整,显示出局部传递的希望.
- 这些微凝通过EPR效应具有癌症治疗的潜力,利用瘤特异性葡萄糖水平.
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