Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Extrinsic and Intrinsic Pathways of Hemostasis01:20

Extrinsic and Intrinsic Pathways of Hemostasis

6.3K
Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
6.3K
Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

636
Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
636

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Clinical predictors of mortality in immune thrombotic thrombocytopenic purpura: A National Inpatient Sample analysis.

British journal of haematology·2026
Same author

Antiphospholipid syndrome (APS) is a platelet factor 4 (PF4)-centric immunothrombotic disorder.

Blood·2026
Same author

Noninvasive Monitoring of Complement Using the Bioluminescent Modified Ham Assay in the Second Porcine-to-Human Live Heart Transplant.

Xenotransplantation·2026
Same author

Bradykinin Contributes to Vasogenic Edema in Murine Experimental Cerebral Malaria.

bioRxiv : the preprint server for biology·2026
Same author

Unmet health care needs in immune thrombotic thrombocytopenic purpura survivors.

Research and practice in thrombosis and haemostasis·2026
Same author

Olfactory Receptor Activation Reduces Platelet Reactivity and Arterial Thrombosis Through Actin Cytoskeleton Remodeling.

Circulation·2026

相关实验视频

Updated: Jun 11, 2025

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
06:29

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

30.5K

补充生物传感器在补充介导的血栓性微血管病变中识别出经典路径刺激.

Michael A Cole1, Nikhil Ranjan1, Gloria F Gerber1

  • 1Division of Hematology, Department of Medicine, Johns Hopkins University, Baltimore, MD.

Blood
|October 2, 2024
PubMed
概括

一种新的基于细胞的生物传感器有助于诊断补充介导的血栓性微血管病变 (CM-TMA). 该方法识别了免疫球蛋白M (IgM) 驱动的补充激活,提供了对疾病机制和潜在治疗点的见解.

更多相关视频

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization
06:28

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization

Published on: June 4, 2020

6.8K
Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
04:37

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

196

相关实验视频

Last Updated: Jun 11, 2025

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
06:29

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

30.5K
Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization
06:28

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization

Published on: June 4, 2020

6.8K
Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
04:37

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

196

科学领域:

  • 免疫学 免疫学 免疫学
  • 血液学 血液学 血液学
  • 细胞生物学 细胞生物学

背景情况:

  • 补充介导的血栓性微血管病变 (CM-TMA),也称为非典型的血清性尿素综合征,是一种严重的疾病.
  • 它与影响补充系统蛋白质和调节者的遗传变异或抗体有关.
  • 当前的诊断方法可能无法完全捕捉所有潜在的机制.

研究的目的:

  • 开发新的基于细胞的补充生物传感器来诊断CM-TMA.
  • 研究不同补充通路在CM-TMA病变发生过程中的作用.
  • 监测补充向疗法的有效性.

主要方法:

  • 工程HEK293细胞缺乏特定的补充调节蛋白 (CD55,CD59,CD46) 被用作生物传感器.
  • 这些生物传感器利用自主生物发光来进行敏感的检测.
  • 用补充路径抑制剂来剖析激活路径.

主要成果:

  • 生物传感器阵列成功检测到CM-TMA中的补体激活.
  • 免疫球蛋白M (IgM) 驱动的经典通路激活在活跃疾病和缓解阶段都被确定.
  • 这一发现为CM-TMA病例提供了解释,而没有确定其他途径变体.

结论:

  • 基于细胞的补充生物传感器为CM-TMA提供了敏感的诊断工具.
  • 这项研究表明,IgM免疫耐受性的崩有助于CM-TMA的一个子集.
  • 这些发现可能有助于开发针对CM-TMA的向疗法.