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通过视网膜酸和甲酸调节人类皮细胞的再生反应

Lídia de Oliveira Fernandes¹, Igor Paulino Mendes Soares², Rafael Antonio de Oliveira Ribeiro²

¹Department of Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, São Paulo, Brazil.

Archives of oral biology

|October 2, 2024

概括

酸和酸生物刺激人类牙纸干细胞 (hSCAPs) 促进再生. 低剂量视网酸和高剂量 Askorbic 酸显示出再生疗法的潜力.

科学领域:

生物材料科学生物材料科学
干细胞生物学干细胞生物学
再生医学是一种再生医学.

背景情况:

来自牙上皮的人类干细胞 (hSCAPs) 对于牙组织再生至关重要.
了解生物活性分子,如视网酸和酸对hSCAPs的影响,对于开发再生疗法至关重要.

研究的目的:

为了研究视网酸和酸对hSCAP活力,原蛋白合成,矿化和基因表达的体外影响.
确定这些化合物的最佳度,以促进hSCAPs中的亲再生表型.

主要方法:

从人类的第三牙中分离出hSCAPs并进行了表征.
细胞接受了不同度的网红酸和甲酸的处理.
试验包括细胞活力 (alamarBlue),原蛋白合成 (Sirius Red),矿化矩阵形成 (Alizarin Red) 和基因表达分析 (RT-qPCR) 矿化,迁移,血管新生和原蛋白合成标志物.

主要成果:

阿斯科布酸 (300μM) 增加了hSCAP的活力,而视网酸则根据剂量而降低了它的活力.
低剂量视网酸 (0.1μM) 和高剂量 Askorbic 酸 (300μM) 增强了矿化矩阵的形成和原蛋白的合成.
基因表达分析显示,两种化合物对矿化,迁移,血管新生和原合成基因的差异调节.

结论:

雷酸 (0.1μM) 和甲酸 (300μM) 有效地生物刺激hSCAPs向一个亲再生的表型.
这些发现表明,在再生性内牙科手术 (REP) 中,网红酸和甲酸的潜在应用.

关键词:

亚斯科布酸是一种亚斯科布酸.牙的乳头是牙的乳头牙纸是一种牙纸.指导组织再生指导组织再生.雷蒂诺酸是什么意思雷蒂诺酸是什么意思特雷蒂诺因 (Tretinoin) 是一种药物.

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