血液体积,空气暴露时间,运输时间和测试延迟对血总二氧化碳的影响在使用微容器模拟的开放采集中
Michael S Reid1, Isolde Seiden Long1, Lawrence de Koning2
1Alberta Precision Laboratories, Diagnostic and Scientific Research Centre, #9 3535 Research Way NW, Calgary, AB T2L 2K8, Canada; Departments of Pathology and Laboratory Medicine, Cumming School of Medicine, Health Sciences Centre, Foothills Campus, University of Calgary, 3030 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.
微容器中的血液体积减少,运输时间更长,测试延迟显著降低总二氧化碳 (TCO2) 水平. 为了确保准确的TCO2测量,请将微型容器完全填满,并立即测试.
科学领域:
- 临床化学 临床化学
- 实验室医学 实验室医学
- 新生儿诊断 新生儿诊断
背景情况:
- 暴露在空气中减少了血总二氧化碳 (TCO2).
- 新生儿在微型中收集血液可能会影响TCO2水平.
- 影响TCO2减少的因素包括采集方法,运输和测试延迟.
研究的目的:
- 评估在微容器中收集的新生儿血液中的TCO2降低.
- 量化微容器体积,空气暴露,运输时间和延迟测试对TCO2.2的影响.
- 在新生儿样本中确定精确的TCO2测量最佳实践.
主要方法:
- 模拟在微型中采集血液,使用3x4的因数设计 (血液体积和空气暴露).
- 医院内外运输时间对全血和离心样本的评估影响.
- 评估的TCO2变化与延迟测试的开放血量使用重复测量ANOVA和学生的T-测试.
主要成果:
- 微容器中的血TCO2比真空容器低3.5 mmol/L.
- 微型中较低的血液体积显著降低了TCO2;空气暴露时间的影响较小.
- 场外运输 (4小时) 和延迟的血测试 (15分钟) 显著降低了TCO2水平.
结论:
- 随着微容器填充量降低,场外运输延长和测试延迟,等离子体TCO2降低.
- 建议包括完全填充微型容器和快速测试,以最大限度地减少TCO2的减少.
- 实验室应考虑这些变量影响的TCO2结果的解释性评论,校正因数或单独的参考间隔.
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