酸酸糖增强肠道微生物群和肠道屏障功能,缓解小鼠中脱氧尼瓦伦醇诱导的宿主损伤
Jinqiu Mi1, Yaoyi Tong1, Qiyue Zhang2
1State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China; Feed Safety and Healthy Livestock, Beijing Jingwa Agricultural Innovation Center, Beijing, China.
The Journal of nutrition
|October 2, 2024
概括
酸寡糖 (AOS) 保护免受脱氧尼瓦伦醇 (DON) 诱导的肠道损伤. AOS改善了肠道屏障功能,增强了有益的肠道细菌,并减少了DON暴露引起的炎症.
科学领域:
- 胃肠病学 胃肠病学
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 酸寡糖 (AOS) 具有抗炎,抗菌和抗氧化特性.
- 脱氧尼瓦伦醇 (DON) 诱导肠道炎症,损害肠道屏障,并破坏微生物群平衡.
研究的目的:
- 在小鼠模型中评估AOS对DON诱导的肠损伤和全身炎症的保护作用.
- 调查AOS对肠道微生物群组成和肠道屏障完整性的影响.
主要方法:
- 小鼠被分为四组:对照组,AOS治疗组,DON暴露组和AOS+DON暴露组.
- 治疗包括每天口服AOS (200 mg/kg BW) 或酸盐缓冲盐水 (PBS) 3周,然后是DON (4.8 mg/kg BW) 或PBS 1周.
- 评估了肠道损伤,屏障功能蛋白,肠道微生物群和短链脂肪酸 (SCFA) 生产.
主要成果:
- DON暴露显著减少了体重和肠的长度,这在一定程度上被AOS缓解了.
- 服用AOS增加了便中的DON度,这表明潜在的结合或改变了代谢.
- DON降低了紧结蛋白 (克劳丁,奥克卢丁) 和粘蛋白2表达,但AOS预治疗改善了这些效应.
- 服用AOS补充剂增加了有益细菌 (Bifidobacterium,Faecalibaculum,Romboutsia) 的丰富性和SCFA的产生 (乙酸盐,酸盐).
结论:
- 酸寡糖 (AOS) 有效地保护免受脱氧尼瓦伦醇 (DON) 诱导的肠损伤.
- AOS增强了肠道微生物群的组成,并加强了肠道屏障功能,提供了潜在的治疗策略来对抗DON毒性.
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