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Circ_0104873通过miR-875-5p/NOTCH3/Notch信号通路促进了骨关节炎的进展

Zhao Tang¹, Wei Zhang², Anlong Liu²

¹Department of orthopedics, Nanjing Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210002, Jiangsu Province, China.

International journal of biological macromolecules

|October 2, 2024

概括

循环RNA circ-IQGAP1 (circ_0104873) 在骨关节炎 (OA) 中过度表达. 抑制circ_0104873通过microRNA-875-5p/NOTCH3通路阻碍骨髓中酶干细胞分化和OA进展.

科学领域:

生物医学科学生物医学科学
分子生物学分子生物学
整形外科整形外科整形外科

背景情况:

骨关节炎 (OA) 是一种普遍的关节疾病,发病率越来越高.
循环RNAs (circRNAs) 正在成为OA病变发生的关键参与者.
大多数circRNAs在OA中的特定作用仍然在很大程度上是未知的.

研究的目的:

研究circ-IQGAP1 (circ_0104873) 在骨关节炎中的作用和机制.
探索circ_0104873对骨髓介质干细胞 (BMSC) 和骨质分化的影响.
阐明涉及OA发展中的circ_0104873的分子途径.

主要方法:

在BMSC骨质生分化的过程中分析circ_0104873表达.
功能性试验,以评估circ_0104873抑制对BMSC增殖和分化的影响.
机制测试以确定与microRNA-875-5p (miR-875-5p) 和NOTCH3.3的相互作用.
救援试验验证了OA中circ_0104873/miR-875-5p/NOTCH3轴的作用.

主要成果:

在BMSC骨质生成差异化过程中,Circ_0104873显著过度表达.
抑制circ_0104873抑制了BMSC的增殖和骨质分化.
发现Circ_0104873对miR-875-5p产生作用,导致NOTCH3的上调和Notch信号通路的激活.
证实Circ_0104873通过准miR-875-5p/NOTCH3轴来促进OA的发展.

结论:

Circ_0104873通过miR-875-5p/NOTCH3/Notch信号通路促进了骨关节炎的进展.
Circ_0104873代表了骨关节炎治疗的潜在治疗标.

关键词:

在NOTCH3中,缺口信号通道的信号通道.骨关节炎是一种骨关节炎.环_010487373 的时间.在 miR-875-5p 中使用.

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