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受感染的伤口修复与通过冷大气等离子体的原I诱导和NOX2激活相关

Océane Blaise^1,2, Constance Duchesne^1,2, Elena Capuzzo¹

¹Institut Pasteur, Université Paris Cité, CNRS UMR6047, Unité de Recherche Yersinia, Paris, France.

NPJ Regenerative medicine

|October 2, 2024

概括

冷大气等离子体 (CAP) 通过激活细胞中的NOX2酶来增强组织修复,从而减少细菌负载并改善伤口愈合. 这项研究阐明了CAP对受感染皮肤损伤的治疗作用背后的机制.

科学领域:

生物医学工程生物医学工程
伤口愈合治愈伤口愈合
血医学是一种血医学.

背景情况:

冷大气等离子体 (CAP) 显示出组织修复和再生医学的前景.
在受感染的皮肤伤口中,CAP的治疗机制尚未完全理解.

研究的目的:

研究 CAP 促进受感染皮肤伤口愈合的分子机制.
确定细胞NADPH氧化酶复合体NOX2在CAP治疗效果中的作用.

主要方法:

在被 Staphylococcus aureus 感染的小鼠皮肤伤口上进行 CAP 治疗.
在巨细胞和生物工程皮肤中对NOX2的遗传和药理抑制.
对细胞内活性氧物种 (ROS),细菌存活率,Rac激活和 I 原表达的分析.

主要成果:

CAP激活了NOX2复合体,增加了细胞内ROS.
在巨细胞和皮肤模型中NOX2抑制导致在CAP治疗下细菌存活率增加.
在小鼠伤口中,CAP治疗减少了细菌负担,增强了新皮肤和细胞外基质形成.
CAP触发了主要NOX2组装蛋白的Rac激活和酸化 (p40^phox,p47^phox).
CAP诱导纤维细胞中的原I表达.

结论:

CAP激活NOX2复合体,有助于感染伤口中的细菌清除.
这些发现揭示了通过CAP促进皮肤修复和感染控制的特定分子通路.

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