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使用PacBio和Hi-C技术组建Astragalus membranaceus的染色体规模的基因组组

Huijie Fan¹, Zhi Chai², Xukui Yang³

¹College of Basic Medical Sciences, Shanxi University of Chinese Medicine, Jinzhong, 030619, China. fanhuijie@sxtcm.edu.cn.

Scientific data

|October 2, 2024

概括

我们介绍了一种染色体规模的基因组组合,用于药用草药Astragalus membranaceus (AM). 这种高质量的基因组将推进Astragali Radix和相关豆类的分子和功能研究.

科学领域:

基因组学就是基因组学.
植物生物学植物生物学
药用化学医学化学

背景情况:

阿斯特拉加勒斯膜状 (鱼类) Bge (AM) 是Leguminosae家族的一种重要的药用草本,用于Astragali Radix.
了解AM的遗传构成对于其药用应用和育种计划至关重要.

研究的目的:

为了生成Astragalus membranaceus的染色体规模的基因组组合.
为分子生物学和AM和其他Leguminosae物种的功能研究提供高质量的基因组资源.

主要方法:

使用Hi-C技术进行染色体规模的基因组组装.
基因组大小估计,结合N50计算,并将组件在伪染色体上.
使用BUSCO评估组装完整性,并分析重复序列.

主要成果:

一个1.43Gb的基因组组合与一个1.67Mb的N50结合,与98.16%定于9个伪染色体.
装配完成率估计为97.27%,LAI为16.22和QV为48.58.
鉴定了29914个编码蛋白质的基因,包括73个在黄路和2048个转录因子中,其67.98%的重复序列.

结论:

一个高质量的染色体规模的基因组组装Astragalus membranaceus已经成功生成.
这种基因组资源显著提高了未来对AM和更广泛的Leguminosae家族的功能基因组研究的潜力.
这些已识别的基因为了解AM中黄类重要化合物的生物合成提供了基础.

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