通过异步糖化和原始玉米粉发酵来研究γ-多聚胺酸的生产
Yutao Gou1, Chenxin Niu1, Fanglan Ge2
1Ren College of life Sciences, Sichuan Normal University, Chengdu, 610101, Sichuan, China.
World journal of microbiology & biotechnology
|October 2, 2024
概括
这项研究引入了一种使用粉通过异步糖化和发酵产生多聚胺酸 (γ-PGA) 的新方法. 这一过程显著提高了g-PGA产量,并为工业应用提供了潜在的成本降低.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
背景情况:
- 粉是生物技术中广泛使用的原材料.
- 粉用于聚聚氨酸 (γ-PGA) 生产的用途尚未被探索.
- 细菌细菌SCP010-1是已知的γ-PGA的生产者.
研究的目的:
- 开发一种新的异步糖化和发酵工艺,用于使用粉生产γ-PGA.
- 优化条件,最大限度地提高粉中的 γ-PGA 产量.
主要方法:
- 使用Bacillus subtilis SCP010-1.实施了一个异步的糖化和发酵策略.
- 优化了粉度,酶剂量 (α-氨基酶,葡萄氨基酶),以及液化和糖化温度.
- 在批量发酵器中发酵了糖化液体.
主要成果:
- 在20%的粉,75U/gα-amylase和72°C的液化过程中,获得了36.31g/L的初始γ-PGA产量.
- 增加了g-PGA产量,达到48.88g/L,具有100U/g的葡萄氨基酶和38h的糖化在60°C.
- 通过对糖化液体的批量发酵,进一步提高了γ-PGA输出量,达到116.08g/L.
结论:
- 新型异步糖化和发酵过程有效地利用粉来生产高产的γ-PGA.
- 这种方法为工业 γ-PGA 制造带来了有前途的进步,潜在的成本降低了 40%.
- 该方法适用于其他基于粉的发酵工业.
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