替代拼接在热应力下动态调节常见鱼胚胎发生.
Shuimu Hu1, Guopeng Tian1, Yulin Bai1
1State Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, China.
BMC genomics
|October 2, 2024
概括
热应激在普通鱼 (Cyprinus carpio) 胚胎发生过程中显著影响替代拼接 (AS). 这项研究揭示了广泛的AS变化,并确定了参与多体鱼体热适应的关键基因.
科学领域:
- 水生生物学 水生生物学
- 基因组学就是基因组学.
- 发育生物学是发展生物学.
背景情况:
- 热压力是影响鱼类发育和生存的关键环境因素.
- 常见的鱼 (Cyprinus carpio) 在胚胎和幼虫阶段容易受到热应激.
- 在热应力下常见鱼胚胎发生过程中,替代拼接的作用尚不清楚.
研究的目的:
- 在不同温度下的普通鱼胚胎发生过程中全面描述替代拼接 (AS).
- 为了确定特定阶段和特定温度的AS基因和事件.
- 阐明普通鱼在AS上的热应力调节机制.
主要方法:
- 在八个发育阶段和四个温度下进行RNA测序 (RNA-seq).
- 特定阶段的AS (SAS) 基因和特定温度的差异表达基因 (TDEG) 的鉴定.
- 对温度特异差异性AS (TDAS) 基因,蛋白与蛋白相互作用 (PPI) 网络,基因本体学 (GO) 和基因和基因组京都百科全书 (KEGG) 途径分析的分析.
主要成果:
- 总共有5,835个SAS基因,21,368个TDEG和2,652个TDAS基因被确定.
- 早期发育阶段 (毛囊,胃囊) 显示对热应激的敏感性增加,AS显著增加.
- TDAS基因主要参与细胞内运输,蛋白质修饰,局部化和生物合成过程;亚基因组B显示出更高的AS活性.
- 确定了关键的TDAS基因 (例如taf2, hnrnpa1, drg2) 和 lncRNAs在热应激反应中的调节作用.
结论:
- 热应激在多个层面上影响普通鱼胚胎发生中的替代拼接调节.
- 这项研究填补了有关AS的知识空白,包括普通鱼的胚胎发生和热应激反应.
- 这些发现有助于了解多体鱼在发育过程中的环境适应机制.
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