相关实验视频

Updated: Apr 11, 2026

Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming

Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming

Published on: July 11, 2011

声波流道使多重流细胞计中的粒子速度延伸成为可能

Yaping Wang¹, Wei Wei¹, Ziyu Han²

¹State Key Laboratory of Precision Measuring Technology & Instruments, Tianjin University, Tianjin 300072, China.

Analytical chemistry

|October 3, 2024

概括

这项研究引入了一种新的声流流动细胞计法,用于低成本的多重生物标志物检测. 该技术使用单个探测器来分析多个蛋白质度,从而实现潜在的现场诊断.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
生物技术是生物技术.

背景情况:

多复合流细胞计对于高通量生物医学和临床分析至关重要.
由于多通道激光器和探测器,传统的流细胞计系统是昂贵和重的,阻碍了小型化.
对于更广泛的应用,需要具有成本效益的小型流细胞计解决方案.

研究的目的:

开发一个小型化的,低成本的多重流细胞计系统.
为了使无聚焦和多尺寸微珠的歧视使用声波流.
使用单个检测器量化多种蛋白质生物标志物.

主要方法:

开发了一种基于声波流动的流动细胞测量技术,用于无微珠聚焦.
在声波流中利用显著的微珠行为来建立一个"速度拉伸"尺寸检测方法.
在急性心肌梗塞样本中进行了心脏热素I,肌酸酶-MB和肌球蛋白的多重检测.

主要成果:

成功演示了多尺寸微珠的无聚焦和尺寸歧视.
建立了一种基于声流诱导的速度差异的新型尺寸检测方法.
通过对心脏生物标志物的多重检测验证了系统的可行性和性能.

结论:

基于声流的流动细胞计能够用单个检测器进行多重检测.
这项技术为低成本,小型化和潜在的现场检测多个生物标志物的途径提供了途径.
开发的系统对治疗点诊断和个性化医疗有着重大影响.

更多相关视频

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Published on: August 27, 2013

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Published on: October 1, 2017

相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...