乳腺癌中体原纤维的几何特征使用差异干扰对比显微镜
Suzan F Ghannam1,2,3, Catrin Sian Rutland3,4, Cinzia Allegrucci3,4
1Academic Unit for Translational Medical Sciences, School of Medicine, University of Nottingham, Nottingham, UK.
Journal of microscopy
|October 3, 2024
概括
乳腺癌 stromal 原蛋白在 in situ 和侵入性阶段之间存在差异. 分析这些原特征可以帮助区分导管癌 in situ 和侵入性瘤,帮助诊断.
科学领域:
- 在瘤学瘤学.
- 生物物理学的生物物理.
- 病理学 病理学 病理学
背景情况:
- 乳腺癌 (BC) 异质性受瘤微环境相互作用的影响.
- 在BC中瘤肌层的诊断和预后意义需要进一步的定义.
- 不同干扰对比 (DIC) 显微镜可提供细胞和组织结构的无标签可视化.
研究的目的:
- 通过DIC显微镜来比较in situ和侵袭性乳腺瘤之间的体原蛋白纤维特性.
- 研究乳腺癌中原体参数的预后价值.
- 开发一个模型来区分在位管道癌 (DCIS) 和侵袭性乳腺癌,基于树突原蛋白特征.
主要方法:
- 使用了200个乳腺癌病例 (100个DCIS,100个侵入性) 和50个验证病例的组织微阵列.
- 在未染色的组织部位上使用DIC显微镜来分析原纤维参数 (方向,对齐,密度,宽度,长度,直度).
- 用血素和素 (H&E) 染色的部分用于组织学审查.
主要成果:
- 与DCIS相比,侵袭性乳腺癌流体表现出更高的原纤维密度.
- 侵袭性BC中的原纤维比DCIS更薄,更直,更无组织,更少对齐.
- 一个基于原特征的预测模型准确地区分了DCIS和侵袭性瘤,准确度为94%.
- 与临床病理学参数和侵袭性乳腺癌患者结果相关的原纤维特性.
结论:
- 在DCIS和侵袭性乳腺癌之间, stromal原纤维结构存在显著差异.
- 在手术前的活检中分析树皮原蛋白可能有助于区分纯粹的DCIS和侵入性疾病.
- 原纤维分析有可能改善乳腺癌的诊断和预后.
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