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相关概念视频

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

2.0K
This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
2.0K
Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

10.0K
In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
10.0K
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

14.5K
The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
14.5K
Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

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This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
9.2K
Primary Culture of Mouse Dopaminergic Neurons11:58

Primary Culture of Mouse Dopaminergic Neurons

39.1K
Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
39.1K
Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

26.4K
Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
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相关实验视频

Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Published on: January 20, 2015

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由于不完整的观测结果的紧张回归,适用于长度研究.

Tianchen Xu1, Kun Chen2, Gen Li3

  • 1Bristol Myers Squibb.

The annals of applied statistics
|October 3, 2024
PubMed
概括

这项研究引入了一种新的方法来分析复杂的纵向微生物群数据,即使是缺失的值. 该方法揭示了婴儿肠道微生物组发育和神经发育结果之间的联系.

科学领域:

  • 生物统计学 生物统计学
  • 微生物组研究 微生物组研究
  • 纵向数据分析 纵向数据分析

背景情况:

  • 多变量纵向数据与各块缺失的值为关联分析带来了重大挑战.
  • 现有的方法经常在不完整的数据集下失败,需要新的分析方法.

研究的目的:

  • 开发一种新的统计方法,将高维,纵向微生物组数据与连续结果关联起来,解决块状缺失问题.
  • 为了利用基于张数的回归来分析复杂的,不完整的纵向数据.

主要方法:

  • 将多变量纵向数据表示为三向张量 (样本-按特征-按时间).
  • 采用一种节的标量对张差回归模型.
  • 开发一种基于共差的规范化估计程序,以处理缺失的数据而不进行归算.

主要成果:

  • 拟议的方法有效地处理纵向数据中块wise缺失的值.
  • 实现了变量选择和时间变化的效应的平稳估计.
  • 在早产婴儿肠道微生物组动态和神经发育之间发现了显著的关联.

结论:

  • 标量对张差回归模型为分析不完整的多变量纵向数据提供了有效的框架.
关键词:
标尺对张力的回归.一个区块的缺失数据.纵向测量 纵向测量 纵向测量微生物组数据的数据

更多相关视频

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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

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相关实验视频

Last Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Published on: January 20, 2015

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Primary Culture of Mouse Dopaminergic Neurons
11:58

Primary Culture of Mouse Dopaminergic Neurons

Published on: September 8, 2014

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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

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  • 该方法提供了关于微生物组动态和婴儿神经发育之间的关系的见解.
  • 该方法通过在微生物组,合成和衰老研究中的应用得到了验证.