通过海洋有氧细菌减少酸盐
Ken Kine1, Shigeki Yamamura2, Seigo Amachi1
1Graduate School of Horticulture, Chiba University, Matsudo, Japan.
Frontiers in microbiology
|October 3, 2024
概括
海洋有氧细菌将酸盐转化为化物,特别是在静态的,氧气有限的条件下. 这一过程涉及缩酶 (Idr) 基因集群,由环境中的水平诱导,影响循环.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物地质化学生物地质化学
背景情况:
- 已知缩酶 (Idr) 基因集群 (idrABP) 是用于无氧环境中的细菌缩酶 (IO3-) 呼吸.
- 尽管它们存在于有氧和无氧物种中,但在有氧细菌中idr基因的功能尚不清楚.
研究的目的:
- 调查海洋有氧细菌具有假定idr基因集群的潜力,以减少酸盐到化 (I-).
- 为了确定氧气可用性和酸盐度对这种还原过程的影响.
- 为了检查在海洋有氧细菌中对暴露的反应中,idr基因的转录调节.
主要方法:
- 在静态和震动条件下用酸盐培养三种海洋有氧细菌 (Roseovarius azorensis,Notoacmeibacter marinus和Aliirseovarius sediminilitoris).
- 通过R. azorensis. 的洗净细胞悬浮来减少酸盐的分析.
- 在R. azorensis中对idrA,idrB,idrP和idrP基因表达进行转录分析 (qRT-PCR).
主要成果:
- 所有测试的细菌在静态 (氧气有限) 条件下显著减少2mM酸盐,但在震动 (有氧) 条件下显示出有限的减少 (0.1-0.5mM).
- 酸盐的减少由R. azorensis依赖于在静态条件下酸盐存在的先前生长.
- 在静态用酸盐培养的R. azorensis中,idrA,idrB,idrP和idrP基因的表达被上调,idrA在0.1μM酸盐诱导后增加了14倍.
结论:
- 海洋有氧细菌可以将酸盐降解为化物,主要是在氧气有限的条件下,这种能力是由环境相关的酸盐度引起的.
- 这些发现表明,海洋有氧细菌在地表水中的化物生产中起着重要作用.
- 这种细菌活动有助于全球循环,并影响大气臭氧水平.
更多相关视频
09:45Laboratory Simulation of an IronII-rich Precambrian Marine Upwelling System to Explore the Growth of Photosynthetic Bacteria
Published on: July 24, 2016
11.5K
08:31Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Published on: October 3, 2018
8.1K
相关概念视频
Oxidation and Reduction of Organic Molecules
8.0K
Energy production within a cell involves many coordinated chemical pathways. Most of these pathways are combinations of oxidation and reduction reactions, which occur at the same time. An oxidation reaction strips an electron from an atom in a compound, and the addition of this electron to another compound is a reduction reaction. Because oxidation and reduction usually occur together, these pairs of reactions are called redox reactions.
The removal of an electron from a molecule, results in a...
The removal of an electron from a molecule, results in a...
8.0K
Redox Titration: Other Oxidizing and Reducing Agents
1.4K
Besides iodine, other oxidizing or reducing agents can serve as titrants in redox titrations. Common oxidizing titrants include KMnO4, cerium(IV), and K2Cr2O7. The choice of oxidizing titrants depends on factors like stability, cost, analyte strength, and reaction rate between the analyte and titrant. KMnO4 is a strong oxidizing titrant that reduces from Mn(VII) to Mn(II) in a highly acidic solution, simultaneously oxidizing the analyte to a higher oxidation state. In this case, KMnO4 acts as a...
1.4K
Metabolism of Chemolithotrophs
1.3K
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
1.3K
Microbial Mats
67
Microbial communities forming biofilms and mats represent complex, spatially structured ecosystems where metabolic processes are stratified according to light, oxygen, and nutrient gradients. Biofilms are initial colonization stages, only a few millimeters thick, while mature microbial mats can reach centimeter-scale thickness and display intricate vertical organization. Their structural and functional heterogeneity allows microorganisms to occupy distinct ecological niches within a few...
67
Microbes and Other Elemental Cycles
89
Microbial activity plays a pivotal role in the biogeochemical cycling of iron and manganese, especially at the redox gradients characteristic of stratified aquatic environments. These cycles are driven by microbial transformations between oxidized and reduced forms of the metals, allowing organisms to exploit them for metabolic energy and structural purposes.Iron Cycling Across Redox GradientsIn neutral, oxygen-rich surface waters, iron is predominantly found in its oxidized, insoluble ferric...
89
Marine Microbial Ecology
66
Marine microbial ecosystems are shaped by distinct physicochemical limits, including high salinity, low nutrient availability, and fluctuating oxygen levels. These conditions favor smaller microbial cell sizes, which maximize their surface-to-volume ratio for efficient nutrient uptake.Microbial activity and community composition are closely linked to biogeochemical cycles, particularly in dynamic environments like estuaries, where halotolerant microbes thrive in response to variable salinity...
66
