相关实验视频
Updated: Jul 5, 2026

09:53
In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
Published on: July 27, 2010
介质素-27调节与控制寄生虫复制相关的自适应性免疫反应 在犬类莱什曼病中
Marilene Oliveira Dos Santos1, Sidnei Ferro Costa1, Gabriela Torres Rebech1
1Department of Clinics, Surgery, and Animal Reproduction, São Paulo State University (UNESP), São Paulo, Brazil.
Parasite immunology
|October 3, 2024
概括
介素27 (IL-27) 在犬类莱什曼病 (CanL) 中起作用. 升高的IL-27亚单元与疾病严重程度相关,可能会损害免疫控制,这表明它是CanL.的治疗点.
科学领域:
- 免疫学 免疫学 免疫学
- 兽医医学 兽医医学 兽医医学
- 寄生虫学的寄生虫学
背景情况:
- 干白素27 (IL-27) 是一种已知的细胞因子,可调节人类和动物模型中的莱什马尼亚婴儿感染易感性.
- IL-27在犬类莱什曼病 (CanL) 中的作用以前没有被研究过.
研究的目的:
- 调查IL-27在犬类莱什曼病期间免疫反应中的潜在调节作用.
- 确定IL-27子单元与CanL.L.中的疾病标志物和寄生虫负担的关联.
主要方法:
- 从CanL受影响和对照犬的脏白细胞培养超级体中测量IL-27子单位 (EBI3和p28).
- 对IL-27亚单位水平与IL-21,抗L.婴儿抗体和寄生虫负载的相关性分析.
- 功能性测试涉及IL-27阻断,以评估其对寄生虫控制,细胞因子生产和免疫细胞标记物的影响 (PD-1,CTLA-4,-Stat-1/3,T-bet,GATA3,NO).
主要成果:
- 与对照组相比,在CanL的狗中,IL-27的两个子单元 (EBI3和p28) 都显著增加.
- IL-27亚单元水平与IL-21,抗L婴儿IgG抗体和寄生虫负载正相关.
- IL-27阻塞实验表明IL-27在上调PD-1,CTLA-4,T-bet和氧化生产中的潜在作用,这表明免疫逃避机制.
结论:
- IL-27和IL-21参与犬类莱什曼病期间的免疫反应.
- IL-27可能会导致宿主免疫系统无法控制CanL中的Leishmania婴儿复制的失败.
- 准IL-27通路可能是治疗犬类莱什曼病的新治疗策略.
相关概念视频
Inflammatory Response
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
T Cell Types and Functions
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Leishmaniasis
Leishmaniasis is a protozoal disease caused by species of the genus Leishmania and transmitted through the bite of infected female sandflies. The parasite exists in two principal morphological forms during its life cycle. A sandfly acquires intracellular amastigotes from an infected reservoir host, such as a dog. Within the sandfly, these forms differentiate into motile, flagellated promastigotes. During a subsequent blood meal, promastigotes are injected into the human host, where they...
Antiprotozoal Agents
Leishmaniasis is a widespread parasitic disease caused by several Leishmania species. It affects millions of people each year and remains a major public health problem in endemic regions. First-line treatment relies on pentavalent antimonials, including meglumine antimoniate and sodium stibogluconate. Even so, how these drugs work has not been fully clear, especially their interaction with parasite-specific biochemical pathways. One key target is trypanothione reductase (TR), an enzyme that...

