鼻息肉中的中性粒细胞表现出转录性适应和益炎作用,这些作用取决于局部的息肉环境
Chen Zhang1,2,3, Qianqian Zhang1,2, Jiani Chen1,2
1ENT Institute and Department of Otorhinolaryngology and.
JCI insight
|October 3, 2024
概括
长期鼻多性鼻炎中的中性粒细胞 (CRSwNP) 适应炎症,释放上素M (OSM). 这种分子增强了免疫反应,在不同的炎症环境中促进了CRSwNP的发展.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 呼吸系统医学 呼吸系统医学
背景情况:
- 带鼻息肉的慢性鼻炎 (CRSwNP) 是一种复杂的炎症性疾病.
- 中性粒细胞是CRSwNP中的关键细胞,但它们在不同的炎症环境中的特定作用尚未完全理解.
研究的目的:
- 研究CRSwNP患者中透到鼻的中性粒细胞的功能性可塑性和转录格局.
- 阐明特定中性粒细胞子集及其分泌因子在CRSwNP病变发生过程中的作用.
主要方法:
- 聚合体透中性粒细胞的综合转录组分析.
- 单细胞RNA测序 (scRNA-seq) 用于识别中性粒细胞子集.
- 流细胞计和体外细胞培养 (上皮细胞,纤维细胞) 来评估中性粒细胞的功能.
主要成果:
- 在CRSwNP中中性粒细胞表现出转录可塑性,形成5个不同的功能子集.
- 在具有IFN-I通路活性的非氨基酸性CRSwNP (neCRSwNP) 中,中性粒细胞5+显著.
- 在CCRSwNP和neCRSwNP中存在的CXCL8+中性粒细胞表现出高激活度和分泌的 Kostatin M (OSM).
- 在鼻上皮质细胞和纤维细胞中,OSM增强了IL-13或IL-17介导的免疫反应.
结论:
- 中性粒细胞在转录上是可塑的,并且在聚的微环境中被激活.
- 中性粒细胞衍生的OSM通过调节表皮和纤维细胞反应,在CRSwNP的发病过程中起着至关重要的作用.
- 了解中性粒细胞异质性和功能对于开发针对CRSwNP的向疗法至关重要.
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