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低度的胆化物会改变牛前植入胚胎的发育计划
McKenzie Lj Haimon1, Eliab Estrada-Cortés2, Thiago Fernandes Amaral3
1M Haimon, Animal Sciences, University of Florida, Gainesville, 32611, United States.
Reproduction & fertility
|October 3, 2024
概括
在早期牛胚胎发育过程中补充胆,即使在生理水平上,也会增强小牛的生长. 来自用胆治疗的胚胎的小牛的断奶体重增加和每日增加,突出了早期生命编程效应.
科学领域:
- 生殖生物学 生殖生物学
- 发展规划是指发展规划.
- 动物科学动物科学
背景情况:
- 胆被认为是胚胎发育的关键营养素.
- 在体外培养过程中高胆度 (1.8 mmol/L) 改变了牛胚胎的发育,影响了产后生长.
- 生理学上相关的胆度对早期胚胎编程的影响尚不清楚.
研究的目的:
- 研究生理学相关的胆度 (4μmol/L) 对牛植入前胚胎发育规划的影响.
- 为了确定血型度的胆是否会影响胚胎发育和随后的小牛表型.
主要方法:
- 采集了牛卵细胞并进行了体外受精.
- 胚胎在补充了4μmol/L胆化物或载体控制 (4μmol/L化) 的介质中培养到囊胞阶段.
- 在试管婴儿中产生的胚胎细胞被转移到同步的接受者中,结果的小牛在出生和断奶时被称重.
主要成果:
- 胆补充剂没有影响芽细胞形成率或怀孕建立.
- 与对照组相比,从用胆治疗的胚胎中出生的小牛的断奶体重显著更高.
- 来自用胆治疗的胚胎的小牛从出生到断奶的每日体重增加有所改善.
结论:
- 胆作为牛前植入胚胎的发育编程剂,即使在生理上相关的度.
- 早期胚胎暴露于胆会影响产后小牛的表型,特别是生长参数.
- 这些发现强调了植入前阶段在确定长期小牛的发育和生产率方面的关键作用.
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