在复杂的生物介质中区分蛋白质冠状病毒和纳米粒子聚合物形成,使用X射线光子相关谱学
Caroline E P Silva1, Agustin S Picco2, Flavia Elisa Galdino1
1Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy & Materials (CNPEM), Campinas, Sao Paulo 13083-970, Brazil.
Nano letters
|October 3, 2024
概括
射线光子相关谱学 (XPCS) 揭示了二氧化纳米粒子 (SiO2) 在复杂的生物流体中表现出一致的布朗运动. 这种技术提供了实时分析,克服了纳米医学应用传统方法的局限性.
科学领域:
- 纳米医学是一种纳米医学.
- 体科学 体科学 体科学
- 生物物理学的生物物理.
背景情况:
- 纳米粒子与生物系统中的生物分子相互作用,导致蛋白质冠状形成和潜在的聚合.
- 了解生物流体中的纳米粒子行为对于纳米医学至关重要.
- 传统方法难以在复杂的生物介质中分析纳米粒子.
研究的目的:
- 通过X射线光子相关谱学 (XPCS) 在各种生物环境中研究二氧化纳米颗粒 (SiO2) 的行为.
- 评估生物介质复杂性和蛋白质冠状形成对纳米粒子动态的影响.
- 为了展示XPCS在实时纳米粒子分析方面的功能.
主要方法:
- 应用X射线光子相关谱学 (XPCS) 来研究二氧化纳米粒子 (SiO2).
- 在一系列生物介质上进行研究,从低到高复杂度.
- 定制纳米粒子表面和介质组成,以区分无冠状系统和蛋白质冠状/聚合物形成.
主要成果:
- 纳米粒子 (SiO2) 始终表现出布朗运动,无论生物介质的复杂性如何.
- 基于表面修饰和介质组成,观察到了纳米粒子行为的差异,区分了无冠状和蛋白质涂层系统.
- XPCS提供了对复杂生物环境中纳米粒子动态的实时见解.
结论:
- XPCS是一种强大的技术,用于实时分析生物介质中的纳米粒子.
- 该研究克服了传统方法在复杂的生物系统中表征纳米粒子行为的局限性.
- 结果为纳米医学应用提供了对体行为和蛋白质冠状形成的更深入的见解.
相关概念视频
X-ray Crystallography
27.1K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
27.1K
X-ray Diffraction of Biological Samples
5.2K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
5.2K
X-ray Imaging
11.1K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
11.1K
Atomic Emission Spectroscopy: Lab
835
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
835
Atomic Fluorescence Spectroscopy
1.2K
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
1.2K
2D NMR: Overview of Heteronuclear Correlation Techniques
930
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
930


