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定量跟踪纳米颗粒在米植物中的物种化和动态

Xiao-Xia Zhou¹, Quanzhi Xiao², Kena Zhang²

¹National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-Environmental Pollution Control and Management, Institute of Eco-Environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China.

Analytical chemistry

|October 3, 2024

概括

研究人员开发了一种新方法来追踪纳米颗粒 (SeNPs) 在米中. 这项研究揭示了SeNP如何在植物中转化和移动,为生物强化提供了洞察力.

科学领域:

环境科学环境科学
植物科学植物科学
分析化学分析化学

背景情况:

由于缺乏有效的方法,很难研究工程纳米粒子 (ENPs) 在植物中的吸收和转化.
纳米颗粒 (SeNPs) 显示出在农业中生物强化的潜力,但它们在植物中的相互作用尚未得到充分理解.

研究的目的:

开发和验证一种用于选择性提取和定量SeNP和水植物中溶解的物种的新方法.
研究米 (Oryza sativa) 中SeNPs的吸收,转移,转化和分泌途径.

主要方法:

用酶消化和膜过来分离SeNPs与大米组织中溶解的.
诱导联等离子体质谱法 (ICPMS) 量化了SeNPs,而与ICPMS (LC-ICPMS) 联的液体染色学分析了溶解的物种 (Se(IV),Se(VI,氨基酸).

主要成果:

该方法实现了SeNP (83.5-91.4%) 和溶解 (73.6-99.4%) 的高回收率.
叶中SeNPs的摄入导致它们转化为化 (Se(IV)) 和有机.
所有物种都向下转移,但只有Se(IV) 和SeNPs通过根部被分泌出来.

结论:

开发的方法有效地区分和量化SeNPs和植物组织中溶解的.
在米中,SeNP经历了转化,它们的转移和分泌途径是特定于物种的.
研究结果提供了关于SeNPs在植物中的命运的关键信息,有助于Se-biofortified作物的开发和理解纳米粒子-植物相互作用.

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