在部区域通过链间的二硫化物键稳定了预制的粉碎二极管
Yu Liu1, Kurt Drickamer1, Maureen E Taylor1
1Department of Life Sciences, Sir Ernst Chain Building, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom.
Glycobiology
|October 3, 2024
概括
巨细胞受体微粒在其部区域通过二硫化物键形成稳定的二聚体,这对于在遇到病原体甘氨酸时启动免疫反应至关重要. 这种理解有助于开发新的治疗策略.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- 具有原结构的巨细胞受体 (Mincle) 是一个关键的模式识别受体. 它可以识别与病原体相关的分子模式,例如mycobacteria上的trehalose五巧克力. 的信号由连接物与其C型碳水化合物识别域 (CRD) 结合启动.
- 据认为,Mincle信号发送的启动涉及受体聚类. 因此,了解Mincle的寡合状态对于阐明其功能至关重要.
- 米克尔与Fc受体马 (FcRγ) 适配分子结合,启动细胞内信号级联.
研究的目的:
- 为了确定Mincle受体的寡合状态.
- 为了确定负责Mincle二元化的特定结构元素.
- 开发一种生产功能MINCLE细胞外域的方法.
主要方法:
- 从转染的哺乳动物细胞中净化Mincle的亲和力.
- 位点定向的突变发生,以删除囊类残留物.
- 化学交叉连接实验. 化学交叉连接实验.
- 使用合成二元化域的细菌表达系统.
主要成果:
- 在哺乳动物细胞中,Mincle存在于预先形成的,与二硫化物结合的二聚体.
- 在子序列中氨酸残留物之间的二硫化键稳定了Mincle二次体.
- 跨膜区域的氨酸残留物对于二次体形成或FcRγ协会并不必不可少.
- 开发了一种有效的协议,用于在细菌中产生与二硫化物相关的Mincle细胞外域.
结论:
- 子通过子区域的二硫化物键形成稳定的二次体,独立于其跨膜域.
- 这种二分化是Mincle在先天免疫力中的作用的关键特征.
- 可以使用细菌表达系统来产生功能Mincle细胞外域,以便进一步研究.
更多相关视频
11:44Synthesis and Structure Determination of µ-Conotoxin PIIIA Isomers with Different Disulfide Connectivities
Published on: October 2, 2018
10.0K
09:37Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
Published on: May 2, 2019
9.7K
相关概念视频
Protein Folding
112.3K
Overview
112.3K
Protein and Protein Structure
71.5K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
A protein's shape is critical to its function. For example, an enzyme...
71.5K
The Structure of Intermediate Filaments
4.5K
The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of microfilaments (7 nm) and the microtubules (25 nm). These filaments are highly stable and can remain intact when exposed to high salt concentrations and detergents. These filaments are responsible for providing stability and mechanical support to the cells. They also help in cell adhesion and maintaining tissue integrity.
Intermediate...
Intermediate...
4.5K
Formation of Intermediate Filaments
3.1K
Intermediate filaments are cytoskeletal proteins with higher tensile strength and flexibility than microfilaments and microtubules. Unlike the other two cytoskeletal proteins, intermediate filament formation lacks the enzymatic activity to hydrolyze nucleotides like ATP and GTP to generate energy for polymerization. Therefore, the formation of intermediate filaments is multistep self-assembly. The involvement of any accessory proteins in intermediate filament formation has not yet been...
3.1K
SNAREs and Membrane Fusion
10.4K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
10.4K
Protein Folding
8.8K
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
8.8K
