巨微RNA-146a是对生物材料植入物异体反应的中央调节者
Manisha Mahanty1, Bidisha Dutta1, Wenquan Ou2
1University of Maryland, Department of Nutrition and Food Science, College Park, MD, 20742, USA.
Biomaterials
|October 3, 2024
概括
向巨细胞中的microRNA-146a (miR-146a) 可以抑制对生物材料的异物反应 (FBR). 这种方法可以通过减少炎症和纤维化来改善植入物生物相容性.
科学领域:
- 生物材料科学 生物材料科学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 生物工程是生物工程.
背景情况:
- 对植入生物材料的异体反应 (FBR) 损害了设备的功能.
- 目前缺乏FBR的治疗方法,需要探索潜在的分子机制.
- 生物材料诱导的FBR涉及复杂的宿主免疫识别和细胞反应.
研究的目的:
- 确定植入生物材料中FBR的关键分子调节器.
- 研究微RNAs (miRs) 和mRNAs在FBR中的作用.
- 探索miR-146a作为调节FBR的潜在治疗标.
主要方法:
- 在小鼠模型中对生物材料植入的反应中分析miR和mRNA表达特征.
- 利用miR-146a的全局和细胞特异性淘汰模型来评估其功能.
- 采用原子力显微镜和引力显微镜来评估组织力学和细胞力.
主要成果:
- miR-146a水平与巨细胞积累,异体巨细胞 (FBGC) 形成和纤维化相反相关.
- 宏菌衍生的miR-146a被证实是FBR和FBGC形成的关键调节者.
- miR-146a调节的基因参与炎症,纤维化和机械感知,影响组织硬性和细胞引力.
结论:
- 大菌miR-146a在编排FBR到生物材料方面发挥着关键作用.
- 调节miR-146a影响关键的细胞过程,如F-actin生产和引力诱导,这对FBGCs至关重要.
- 向巨细胞miR-146a是一个有希望的策略,通过抑制FBR来增强生物材料的生物相容性.
相关概念视频
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
02:39lncRNA - Long Non-coding RNAs
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01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
01:29Translational Regulation
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...