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辅助RNA转录的通用CRISPR/Cas12a系统用于对多种结直肠癌相关的microRNA进行可编程分析

Xia Cheng¹, Wenchen Zhao², Dandan Ren³

¹Department of General Surgery, Huadong Hospital, Fudan University, No.221 West Yan'an Road, Shanghai, 200040, China; Department of Pharmacy, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.

|October 3, 2024

概括

一个新的生物传感器准确地检测到与结直肠癌相关的多个微RNA (miRNA). 这种先进的系统提供了一个敏感和特定的工具,用于进行非侵入性早期癌症诊断,并区分癌症和正常组织.

科学领域:

生物技术是生物技术.
分子诊断学分子诊断
在瘤学瘤学.

背景情况:

准确的,非侵入性的微RNA (miRNA) 分析对于早期结直肠癌 (CRC) 诊断至关重要.
低miRNA丰度和高序列同质性带来了重要的检测挑战.

研究的目的:

开发一种通用和可编程的诊断策略,同时检测多个CRC相关的miRNAs.
实现高度敏感和特定的miRNA检测,以改善癌症诊断.

主要方法:

与CRISPR/Cas12a系统相结合的顺序可编程滚动圆转录 (RCT).
利用Cas12a的高跨裂变活性进行敏感的目标miRNA检测.
验证了检测四种特定miRNAs (miR-21,miR-17,miR-31,miR-92a) 的系统.

主要成果:

目标miRNA的低检测极限 (LOD) 已达到 (例如,miR-92a的1.0 pM).
成功地区分了正常的结肠上皮细胞 (NCM460) 和结肠癌细胞 (HT29).
在区分准癌和结直肠癌组织方面表现出卓越的能力.

结论:

通用RCT-Cas12a策略为miRNA检测提供了一个可扩展,快速和特定的方法.
这种可编程生物传感器对结直肠癌研究和临床应用具有巨大的前景.
为复杂的诊断挑战提供了基于CRISPR的现有系统的潜在替代方案.

关键词:

这就是CRISPR/Cas12a.结肠直肠癌是一种癌症.微RNA的确定方法滚动圈的转录滚动圈的转录信号放大信号放大

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