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暴露于U(VI) 对肺上皮细胞BEAS-2B细胞的生物学影响

Mingxia Zhang¹, Han Zhou², Lei Liu³

¹College of Life Sciences, Anhui Normal University, Wuhu 241000, China; Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.

|October 3, 2024

概括

(VI) 暴露会导致肺上皮细胞受到显著损伤,包括DNA损伤和抑制增殖. 这项研究详细介绍了由毒性破坏的分子通路.

科学领域:

毒理学毒理学毒理学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

(VI) 是一种具有已知的健康风险的环境毒素.
肺上皮细胞是吸入毒素的主要目标.
了解对细胞的影响对于风险评估至关重要.

研究的目的:

研究 (VI) 对肺上皮细胞的生物作用.
阐明引起的细胞毒性背后的分子机制.
为了确定受U (VI) 暴露影响的关键信号通路.

主要方法:

对细胞活力的MTT测定.
免疫光和流细胞测量用于细胞应激和DNA损伤.
西方涂抹用于分析蛋白质表达和信号通路 (MAPK,PI3K/AKT/GSK-3β/β-catenin).

主要成果:

U(VI) 暴露诱导了氧化应激,炎症,并激活了MAPK通路.
观察到显著的细胞膜损伤和DNA损伤.
通过破坏PI3K/AKT/GSK-3β/β-catenin通路并降低CyclinD1表达,U(VI) 抑制了细胞的增殖和迁移.
细胞周期延迟,线粒体损伤和能量代谢降低被注意到.

结论:

(VI) 对肺上皮细胞产生显著的细胞毒性作用.
分子干扰包括氧化应激,DNA损伤和细胞循环调节的改变.
PI3K/AKT/GSK-3β/β-catenin通路是肺细胞中U(VI) 毒性的关键目标.

关键词:

在BEAS-2B细胞中,细胞循环的细胞周期细胞毒性细胞毒性 U (((VI) 的意思是说.

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