VvRF2b与VvTOR相互作用,并影响葡萄中VvTOR调节的糖代谢
Shuang Xia1, Ying Zhao2, Qiaoyun Deng1
1Beijing Key Laboratory of Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
研究人员发现了VvRF2b,这是一种转录因子,可以负面调节葡萄酒中糖的积累. 它的淘汰会增加糖分水平,并与拉帕素的目标 (TOR) 途径相互作用,影响葡萄的质量.
科学领域:
- 植物分子生物学 植物分子生物学
- 葡萄藤 (Vitis vinifera) 的生理学和生理学
- 糖代谢的生物化学
背景情况:
- 葡萄的品质,对于酒至关重要,受到糖分的重要影响.
- 拉帕素 (TOR) 激酶的向位途径与葡萄中糖代谢的调节有关.
- 控制葡萄葡萄中的糖累积的关键转录因子在很大程度上仍未被描述.
研究的目的:
- 为了确定参与Vitis vinifera糖代谢的新型转录因子.
- 阐明bZIP转录因子VvRF2b在糖累积中的作用.
- 研究葡萄发育中的VvRF2b与TOR信号通路之间的相互作用.
主要方法:
- 使用CRISPR/Cas9基因淘汰 (ko) 和过度表达 (oe) 的转基因"卡伯内特索维尼翁"葡萄.
- 针对VvRF2b.进行亚细胞局部化和转录激活试验.
- 在葡萄果发育过程中分析VvRF2b和VvTOR基因表达和糖含量 (葡萄糖,果糖,糖糖).
- 生物分子光补充和光酶补充试验以确认蛋白质-蛋白质相互作用.
主要成果:
- VvRF2b淘汰 (koVvRF2b) 导致果糖和糖的积累增加,确定VvRF2b是糖的负调节者.
- VvRF2b 作为一种在细胞核和细胞膜中局部化的转录激活剂.
- VvRF2b与VvTOR蛋白相互作用,koVvRF2b增强了VvTOR介导的糖积累和基因表达在OeVvTOR中.
结论:
- VvRF2b在抑制葡萄酒中的糖累积方面发挥着至关重要的作用.
- VvRF2b与TOR蛋白相互作用,影响TOR调节的糖代谢.
- 这种互动为控制葡萄糖含量和质量的复杂监管网络提供了新的见解.
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