CPX-351的细胞吸收通过掠食者受体类B型1介导的非内分细胞通路
Hiroaki Araie1, Naoko Hosono1, Takahiro Yamauchi1
1Department of Hematology and Oncology, Faculty of Medical Sciences, University of Fukui, Fukui, Japan.
像CPX-351这样的脂质体上的蛋白质冠状体会通过清除器受体类B型1 (SR-BI) 影响白血病细胞的吸收. 用BLT-1准SR-BI可能会提高CPX-351的癌症治疗疗效.
科学领域:
- 纳米医学和药物输送
- 细胞生物学和生物化学
- 癌症治疗方法 癌症治疗方法
背景情况:
- 脂质体药物吸收到细胞中对于治疗成功至关重要.
- 脂质体上的蛋白冠状体 (PC) 影响细胞相互作用和吸收通路.
- CPX-351是一种脂质体配方的细胞arabine 和 daunorubicin 用于白血病治疗.
研究的目的:
- 为了研究CPX-351.1的蛋白冠状组成.
- 为了阐明CPX-351在白血病细胞中的吸收途径.
- 探索清除器受体类B型1 (SR-BI) 在CPX-351吸收中的作用及其治疗潜力.
主要方法:
- 使用纳米流液体染色学-并联质谱学分析CPX-351的蛋白冠状分析.
- 通过流细胞计量对白血病细胞系 (HL-60,K562,THP-1) 中的CPX-351摄取量进行量化.
- 使用克拉特林介导的内分细胞抑制剂,巨细胞抑制剂和SR-BI抑制剂BLT-1的抑制研究.
主要成果:
- 脂蛋白A-I和A-II是CPX-351PC的主要组成部分,与SR-BI结合.
- SR-BI表达与CPX-351吸收相关,这种吸收通过抑制内细胞和巨细胞减少.
- 低剂量的BLT-1降低了吸收,而高剂量的BLT-1增加了吸收,这表明巨型皮诺细胞的激活.
结论:
- CPX-351的吸收由SR-BI调解,并受到内细胞通路的影响.
- 高剂量的BLT-1通过巨型皮诺细胞体增强了CPX-351的吸收,这可能会增加治疗疗效.
- SR-BI可以作为CPX-351治疗的生物标志物,组合治疗可能是有益的.
更多相关视频
07:41A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
Published on: February 20, 2018
12:48Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
Published on: August 21, 2017
相关概念视频
Receptor-mediated Endocytosis
Delivery Pathways to the Lysosome
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
ER Retrieval Pathway
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
Carrier-Mediated Transport
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
Mechanisms of Drug Absorption: Paracellular, Transcellular, and Vesicular Transport
However, most drugs use the transcellular route, traversing directly through the cell membranes via two mechanisms: passive and active transport. Passive...
Clathrin Coated Vesicles
