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Updated: Aug 12, 2026

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
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最近在表达查和样本评估方面取得的进展,用于对膜蛋白的结构研究

Yichen Huang1, Ziyi Zhang1, Motoyuki Hattori1

  • 1State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Neurobiology, School of Life Sciences, Fudan University, Shanghai 200438, China.

Journal of molecular biology
|October 3, 2024
PubMed
概括

光检测大小排除色谱 (FSEC) 快速选膜蛋白质进行结构研究,克服表达和稳定性挑战. 这种方法可以加速结构和功能分析,即使使用冷电子显微镜 (cryo-EM).

关键词:
表达方式表达方式表达膜蛋白是一种膜蛋白.查检查 查检查 查检查结构生物学是结构生物学.

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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