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Updated: Jun 11, 2025

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在Actinobacteria中进行金门克隆:机遇和挑战

Anna Christina R Ngo1, Melody Haarmann1, Nils Weindorf1

  • 1Microbial Biotechnology, Ruhr University Bochum, Bochum, Germany.

Methods in molecular biology (Clifton, N.J.)
|October 3, 2024
PubMed
概括

我们介绍了高效的黄金门克隆方法,用于Actinobacteria,使得DNA结构的无组装能够用于生物工程. 这一策略在这些重要的微生物中促进了异质基因表达.

关键词:
行动性细菌 (Actinobacteria) 是一种细菌.电穿孔是一种电子穿孔.在金门大会上,金门门大会举行.罗多科库斯 (Rhodococcus) 是一种植物.

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Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
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