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来自SARS-CoV-2的细胞侵入性粉样组件可以形成多重多态,具有不同的神经毒性

Oana Sanislav¹, Rina Tetaj^2,3, Metali³

¹Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Melbourne, Victoria 3086, Australia.

|October 4, 2024

概括

在 COVID-19 疫情中,

科学领域:

神经科学是一个神经科学.
生物化学生物化学
病毒学病毒学

背景情况:

COVID-19的神经症状 (神经COVID) 可能会持续,导致COVID-19后急性连续性 (PASC).
在神经COVID和PASC中涉及到SARS-CoV-2蛋白质碎片自我组装成粉状纳米纤维.
之前的研究发现了ORF6和ORF10碎片,形成了神经毒性粉样蛋白组合.

研究的目的:

研究SARS-CoV-2ORF6和ORF10碎片的自我组装机制和纳米架构.
确定这些病毒性粉样蛋白组合的生物反应,包括神经毒性.
探索粉样多态化在神经COVID病变发生中的作用.

主要方法:

使用化溶剂溶解SARS-CoV-2ORF6和ORF10,防止已经存在的聚合物.
描述了自组装机制和纳米架构,包括带和无形聚合物.
评估了神经毒性和线粒体呼吸对培养神经元的影响.
提供了细胞吸收病毒性粉样蛋白的直接证据.

主要成果:

在化溶剂中的溶解使组件转向更高的自由能量结构 (丝带,无形聚合物).
这些更高的自由能量组合对培养的神经元具有较低的毒性,但影响了线粒体呼吸.
证明了病毒性粉样蛋白细胞吸收的第一个直接证据.
突出了粉样多态和神经毒性之间的相关性.

结论:

了解病毒粉样蛋白多态性对于理解神经COVID和PASC至关重要.
病毒性粉样蛋白的纳米架构和多态景观影响了它们的神经毒性潜力.
病毒性粉样蛋白可以被细胞内化,导致神经功能障碍.

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