乳酸细菌产生的α-谷氨酸抑制了 hialuronidase 的活性
Taiki Sato1, Takahiro Matsuda2, Keisuke Tagawa1
1Development Laboratories, Nissin York Co., Ltd., 272 Kamimurakimi, Hanyu, Saitama 348-8549, Japan.
Bioscience of microbiota, food and health
|October 4, 2024
概括
研究人员确定了alpha-ketoglutarate (AKG),一种乳酸细菌 (LAB) 的代谢物,作为一种强大的氨酸酶抑制剂. 这一发现为开发抗衰老护肤产品和功能性食品提供了一种新的方法.
科学领域:
- 生物化学 生物化学
- 皮肤病学 皮肤病学
- 食品科学 食品科学 食品科学
背景情况:
- 日本人口老龄化推动了对抗老龄化护肤品的需求.
- 紫外线辐射触发的氨酶激活会降解氨酸,导致皮肤松.
- 乳酸细菌 (LAB) 发酵食品表现出 hialuronidase 抑制作用,但活性化合物未知.
研究的目的:
- 为了阐明LAB介导的 hialuronidase 抑制的机制.
- 为了确定LAB发酵饮料中负责抑制氨酸酶的特定化合物.
- 评估这些化合物在抗衰老应用中的潜力.
主要方法:
- 在LAB发酵牛奶饮料超水体中测试氨酸酶抑制的测试.
- 使用疏水吸附剂进行净化,并通过高性能液态染色学进行分离.
- 使用液态色谱仪飞行时间质谱法识别抑制化合物.
主要成果:
- 阿尔法谷氨酸 (AKG) 被确定为主要的氨酸酶抑制剂.
- AKG表现出强大的抑制作用,其IC50大约是二染糖酸盐的0.13倍.
- 乳杆菌酸性菌JCM1132被确定为一个高AKG生产者 (63.9μg/mL).
结论:
- 这项研究是首次报告AKG,LAB代谢物的一种氨基酶抑制.
- AKG为抗衰老护肤和功能性食品提供了一个有前途的天然成分.
- 这些发现支持开发利用AKG的新型化品和营养保健品.
相关概念视频
Hydrolysis
104.6K
Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
104.6K
Alkylation of β-Diester Enolates: Malonic Ester Synthesis
3.3K
Malonic ester synthesis is a method to obtain α substituted carboxylic acids from ꞵ-diesters such as diethyl malonate and alkyl halides.
3.3K
Preparation of Aldehydes and Ketones from Nitriles and Carboxylic Acids
3.4K
Although it is possible to reduce a carboxylic acid to an aldehyde, strong reducing agents, like lithium aluminum hydride (LAH), prohibit a controlled reduction, instead causing the generated aldehyde to instantly over-reduce to a primary alcohol.
Reducing carboxylic acid derivatives like acyl chlorides (RCOCl), esters (RCO2R′), and nitriles (RCN) using milder aluminum hydride agents like lithium tri-tert-butoxyaluminum hydride [LiAlH(O-t-Bu)3] and diisobutylaluminum hydride [DIBAL-H]...
Reducing carboxylic acid derivatives like acyl chlorides (RCOCl), esters (RCO2R′), and nitriles (RCN) using milder aluminum hydride agents like lithium tri-tert-butoxyaluminum hydride [LiAlH(O-t-Bu)3] and diisobutylaluminum hydride [DIBAL-H]...
3.4K
Fates of Pyruvate
8.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.4K
Acid Halides to Carboxylic Acids: Hydrolysis
2.6K
Hydrolysis of acid halides is a nucleophilic acyl substitution reaction in which acid halides react with water to give carboxylic acids. The reaction occurs readily and does not require acid or a base catalyst.
As shown below, the mechanism involves a nucleophilic attack by water at the carbonyl carbon to form a tetrahedral intermediate. This is followed by the reformation of the carbon–oxygen π bond along with the departure of a halide ion. A final proton transfer step yields carboxylic...
As shown below, the mechanism involves a nucleophilic attack by water at the carbonyl carbon to form a tetrahedral intermediate. This is followed by the reformation of the carbon–oxygen π bond along with the departure of a halide ion. A final proton transfer step yields carboxylic...
2.6K


