调查疑似巨细胞动脉炎和多肌痛类风湿症患者的I型干扰素反应
Marieke van Nieuwland1,2, A H Leontine Mulder3,4, Edgar M Colin1
1Department of Rheumatology and Clinical Immunology, Hospital Group Twente (Ziekenhuisgroep Twente), Almelo, The Netherlands.
干扰素I型 (IFN-I) 标记和血清标记不能可靠地区分巨细胞关节炎 (GCA) 和多肌痛风湿 (PMR) 患者与对照者,这表明诊断效用有限.
科学领域:
- 类风湿病学 类风湿病学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 巨细胞动脉炎 (GCA) 和多肌痛性风湿 (PMR) 是相关的炎症状况.
- 确定活跃疾病的可靠生物标志物和指导治疗至关重要.
- 干扰素I型 (IFN-I) 在GCA和PMR病原发生中的作用需要进一步研究.
研究的目的:
- 在GCA和PMR患者中探索全身IFN-I特征和IFN-I诱导的血清标记物.
- 为了确定这些标记能否区分患者和对照人群,或表明需要治疗.
主要方法:
- 定量逆转录聚合酶连锁反应 (RT-qPCR) 用于测量外围血液单核细胞中的IFN-I刺激基因.
- 计算了一个IFN-I得分.
- 使用多重免疫试验测量IFN-I诱导的CXCL10和galactin-9的血清水平.
主要成果:
- 在GCA,PMR和对照组之间没有发现IFN-I得分的显著差异.
- 在小比例的GCA和PMR患者中检测到IFN-I签名,但不是对照组.
- 与对照组相比,GCA或PMR患者的血清CXCL10和galactin-9水平没有升高.
结论:
- 系统性IFN-I签名不能可靠地将GCA和PMR患者与对照区分开来.
- 像CXCL10和galactin-9这样的IFN-I诱导的血清标记在GCA和PMR中没有上调.
- 易于测量的IFN-I诱导血清标记不太可能有助于诊断或选择治疗GCA和PMR.
更多相关视频
10:00High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
08:26Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Published on: June 14, 2018
相关概念视频
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF
Myocarditis III: Medical Management
Myasthenia Gravis: Overview and Treatment
These antibodies interfere with the function of the nicotinic receptors in three ways: by binding to the receptor and disrupting acetylcholine binding; by causing cross-linking of receptors which...
Inflammatory Response I: Vascular and Cellular
Myocarditis II: Clinical features and Diagnostic Tests
