关于TEER (跨表皮/内皮电阻) 测定 (MIRTA) 报告的最低信息
Monita Sharma1, Erin Huber2, Emma Arnesdotter3
1PETA Science Consortium International e.V., 70499, Stuttgart, Germany. monitas@thepsci.eu.
Archives of toxicology
|October 4, 2024
概括
标准化报告对于跨上皮/内皮电阻 (TEER) 测定至关重要. 关于TEER测定报告的新最低信息 (MIRTA) 建议提高了毒性测试中的数据透明度和可重复性.
科学领域:
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
- 测试开发 测试开发
背景情况:
- 超上皮/内皮电阻 (TEER) 测定对于评估细胞屏障完整性和毒性至关重要.
- 不一致的报告阻碍了实验室间的比较和数据解释.
研究的目的:
- 建立关于TEER试验 (MIRTA) 报告的最低信息建议.
- 提高TEER试验报告的一致性,透明度和可重复性.
主要方法:
- 一个国际合作 (RespTox Collaborative) 审查了来自十二个实验室的TEER测试协议.
- 分析了协议,以确定常见的步骤,实验室间的变异以及它们对结果的影响.
主要成果:
- 为标准化TEER试验报告制定了MIRTA建议.
- 确定了TEER测量的关键参数和潜在变化来源.
结论:
- MIRTA的建议将提高数据的透明度,可重复性,并促进跨实验室比较.
- 这些指导方针可以适应各种细胞屏障系统,并帮助监管机构接受.
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