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人体肠道乳酸细菌的抗菌和抗菌膜活性
Sherin Joy Parappilly1, E K Radhakrishnan2, Sumi Mary George3
1Post Graduate and Research Department of Microbiology, Sree Sankara College, Kalady, 683574, Kerala, India.
概括
新型乳酸细菌 (LAB) 和它们的联盟 (LABCON) 显示出强大的抗菌和抗菌膜对肠道病原体的影响. 这些发现突出了LABCON.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 传染性疾病 传染性疾病
背景情况:
- 肠病原菌如大肠杆菌和金黄色葡萄球菌 (Staphylococcus aureus) 构成了重大的公共卫生挑战.
- 抗生素耐药性需要探索替代抗菌战略.
- 乳酸细菌 (LAB) 以其潜在的益生菌和抗菌素特性而闻名.
研究的目的:
- 研究新型乳酸细菌 (LAB) 菌株及其联盟 (LABCON) 的抗菌和抗菌膜活性.
- 评估LAB和LABCON对关键肠病原菌的疗效,特别是对大肠杆菌 (ATCC 35218) 和金黄色葡萄球菌 (ATCC 25923) 的疗效.
- 确定LAB产生的特定抗微生物化合物,负责抑制细菌生长和生物膜形成.
主要方法:
- 从健康的人类志愿者中分离和描述新的LAB菌株.
- 从LAB分离物及其联盟 (LABCON) 制备甲醇提取物.
- 使用标准微生物分析评估抗菌活性.
- 使用聚焦拉曼显微镜,原子力显微镜 (AFM) 和光显微镜评估抗菌膜活性.
- 鉴定LAB产生的抗微生物化合物,包括DL-3 酸,DL-p-基酸和酸.
主要成果:
- 在LAB分离物和LABCON的甲醇提取物中,针对大肠杆菌 (ATCC 35218) 和金黄色葡萄球菌 (ATCC 25923) 显示出显著的抗菌活性.
- 实验室和LABCON表现出强大的抗菌膜活性对测试的肠病原体.
- 鉴定到的化合物DL-3酸,DL-p-基酸和酸与观察到的抗菌作用有关.
- 同焦拉曼显微镜,AFM和光显微镜为抗菌膜机制提供了详细的见解.
结论:
- 与个人LAB分离物相比,LABCON财团显示出优越的抗微生物和抗菌膜疗效.
- 由LAB产生的已识别的化合物在抑制细菌生长和生物膜形成方面发挥着至关重要的作用.
- 作为一种用于感染控制的天然剂,LABCON具有显著的前景,特别是针对大肠杆菌和金黄色杆菌.
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