将向性遗传标记物整合到基因型测序中,为最终的基因型测序工具提供最终的基因型测序工具
Maxime de Ronne1,2,3, Amina Abed4, Gaétan Légaré2
1Département de Phytologie, Université Laval, Québec, Canada.
概括
一个新的全基因组和有针对性的Amplicon (GTA) 基因型化平台将有针对性的标记物集成到基因型测序 (GBS) 库中. 这种具有成本效益的解决方案可以在育种计划中实现标记辅助选择 (MAS) 和基因组选择 (GS).
科学领域:
- 植物育种 植物育种
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 标记器辅助选择 (MAS) 和基因组选择 (GS) 在植物育种中越来越多地使用.
- 像基因型测序 (GBS) 这样的高通量基因型化方法对于全基因组分析具有成本效益,但不能对基因型向标记进行基因型化.
- 针对性方法,如安普利康序列测序 (AmpSeq),在全基因组应用中受到成本的限制.
研究的目的:
- 开发一种创新且具有成本效益的植物育种基因定型解决方案.
- 将目标标记物基因型化与全基因组基因型化方法相结合.
- 为满足MAS和GS标志物要求提供单一测试.
主要方法:
- 开发了全基因组和向安普利康 (GTA) 基因型化平台.
- 集成的多重目标安普利康集成到GBS图书馆准备中.
- 专为大豆和大麦中的高价值标记物设计的定制原料.
- 推出了Fast-GBS.v3,一个用于数据分析的生物信息管道.
主要成果:
- 在GTA平台上,可以同时从同一样本中进行GBS和向基因型鉴定.
- 实现了GBS和向基因型数据的高效和成本效益的生产.
- 成功准大豆中的23个标记物和大麦中的36个标记物.
- 快速-GBS.v3管道为GTA库提供了全面的数据输出.
结论:
- 该GTA平台为育种者和研究人员提供了一个全在一体的,具有成本效益的基因造型解决方案.
- 这种高通量,低成本的方法促进了DNA标记物在育种计划中的应用.
- 综合方法支持单个试验中MAS和GS标志物要求.
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