在转录后的环境中,微生物组诱导的重编程使用纳米孔直接RNA测序
Zihe Xu1, Xiaoqi Zheng1, Jiajun Fan2
1Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; School of Life Sciences, South China Normal University, Guangzhou 510631, China.
Cell reports
|October 4, 2024
概括
微生物组在全球范围内影响宿主基因表达,影响RNA修饰和大脑和脑中的转录水平. 这项研究揭示了微生物如何通过组织特异性调节过程重新编程宿主基因表达.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 微生物群显著影响宿主生理和基因表达.
- 详细研究由微生物群调节的宿主转录格局的全面研究是有限的.
研究的目的:
- 综合检查由小鼠大脑和皮组织中的微生物组调节的宿主mRNA景观.
- 为了阐明微生物群对RNA修饰,异形生成,多A尾巴长度和转录丰度的影响.
主要方法:
- 利用纳米孔直接RNA测序来分析mRNA景观.
- 对比无特定病原体和无细菌的小鼠,以评估微生物群的影响.
- 研究的RNA修饰包括m6A,m5C和 Ψ.
主要成果:
- 微生物组在全球范围内影响RNA修饰,异形生成,多种类型的尾巴长度,以及大脑和体中的转录丰度.
- 微生物组对转录后调节的影响是组织特异性的.
- 微生物组影响宿主组织中多个RNA修饰的协调.
结论:
- 建立了微生物调节和宿主基因表达之间的明确关系.
- 提供了对微生物组重编程宿主基因表达的机制的见解.
- 突出了微生物群对宿主转录格局的全球和组织特异性影响.
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