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在子宫内膜上皮细胞中失调的miR-124-3p通过改变极性和粘附来降低子宫内膜受容性

Wei Zhou^1,2, Michelle Van Sinderen³, Katarzyna Rainczuk³

¹Department of Obstetrics, Gynaecology and Newborn Health, University of Melbourne, Parkville, VIC 3010, Australia.

Proceedings of the National Academy of Sciences of the United States of America

|October 4, 2024

概括

在子宫内膜中MicroRNA-124-3p的升高会通过破坏细胞粘附和极性而损害胚胎植入. 在不育妇女中减少这种microRNA.

科学领域:

生殖生物学生殖生物学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

异常的子宫内膜受容性是不孕不育和胚胎植入失败的关键原因.
在患有慢性子宫内膜炎的不孕妇女中,MicroRNA-124-3p的水平升高,并影响细胞粘附.
子宫内膜受容性需要精确调节细胞粘附和极性,以便胚胎植入.

研究的目的:

研究微RNA-124-3p在调节子宫内皮细胞粘附和胚胎植入中的功能作用.
确定microRNA-124-3p对子宫内膜受体性和不孕能力的影响.

主要方法:

使用可诱导,子宫表皮特异性微RNA过度表达的小鼠模型.
检查了从妇女的子宫内膜组织中检查的microRNA-124-3p水平,原因不明的不孕.
使用初级人类子宫内膜上皮细胞 (HEEC) 的3D培养模型来评估胚胎附着和细胞行为.

主要成果:

在小鼠模型中,微RNA-124-3p的升高损害了子宫内膜受体性,并导致植入失败.
在接受窗口期间,不孕妇女的子宫内膜中MicroRNA-124-3p异常增加.
在人体细胞中过度表达microRNA-124-3p减少了胚胎的附着和细胞极性受损;减少它使粘附正常化.

结论:

微RNA-124-3p通过调节细胞粘附和极性,在调节子宫内膜受体性方面发挥着至关重要的作用.
异常的microRNA-124-3p水平有助于胚胎植入失败和不孕.
微RNA-124-3p及其点IQGAP1是改善子宫内膜接受力的潜在治疗点.

关键词:

胚胎植入胚胎植入宫内膜上皮质的内膜上皮质.宫内膜的受体性宫内膜的受体性在 miR-124-3p 中使用.这是一个微型RNA.

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