功能修饰的石墨烯氧化物作为一种替代的纳米载体,用于增强dSRNA传递,以改善基于RNAi的昆虫害虫控制
Qi Xue1, Jiangjie Li1, Sven Vereecken2
1Molecular Entomology Laboratory, Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent 9000, Belgium.
Journal of agricultural and food chemistry
|October 4, 2024
概括
石墨烯氧化物纳米载体有效地传递双链RNA (dsRNA) 用于RNA干扰 (RNAi) 害虫控制. 这些生物安全的纳米载体增强RNAi有效性和昆虫死亡率,细胞毒性最小.
科学领域:
- 生物技术是生物技术.
- 纳米技术 纳米技术
- 昆虫学 昆虫学是一门学科.
背景情况:
- RNA干扰 (RNAi) 为害虫管理提供了一种可持续的方法.
- 有效的RNAi需要对双链RNA (dsRNA) 有效的传递系统.
- 纳米载体对于增强dsRNA稳定性和细胞吸收至关重要.
研究的目的:
- 开发和评估石墨烯氧化物纳米载体 (GONs) 用于dSRNA传递.
- 评估GONs-dsRNA复合体的稳定性和细胞吸收.
- 在昆虫害虫模型中研究GONs介导RNAi的疗效.
主要方法:
- 石墨烯氧化物被改造成正电荷纳米载体 (GON).
- GONs与针对真空ATPase基因的dsRNA复杂化 (vha26).
- 评估了稳定性,细胞吸收,基因淘汰,昆虫死亡率和细胞毒性.
主要成果:
- GONs与dsRNA形成稳定的复合体 (106 nm),在各种条件下有效.
- GONs促进了dSRNA向昆虫细胞的传递,增强了目标转录敲击.
- 在*D. suzukii*中,GONs@dsRNA诱导了显著的死亡率和基因敲击,但不是在*S. exigua*.
- 在细胞和生物体水平上,GONs表现出微不足道的细胞毒性.
结论:
- GON是有效的和生物安全的纳米载体,用于昆虫的dSRNA传递.
- 这项技术推动了用于害虫控制和基础研究的RNAi应用.
- 农业保护区为可持续的害虫管理提供了一个有希望的替代策略.
相关概念视频
02:15Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
01:23RNA Interference
26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
02:30siRNA - Small Interfering RNAs
16.6K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.6K