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大的纳米粒子如何将小的纳米粒子带入细胞:通过传输电子显微镜捕获的动作

Xue-Rui Tang¹, Shou-Yang Lei¹, Qiangqiang Zhang¹

¹Institute of Nanochemistry and Nanobiology, Shanghai University, Shanghai 200444, China.

Colloids and surfaces. B, Biointerfaces

|October 4, 2024

概括

较大的纳米颗粒 (100nm) 通过内细胞分裂触发细胞吸收较小的 (50nm). 这种相互作用增强了纳米粒子的传递,并对纳米医学和安全评估产生影响.

科学领域:

纳米技术纳米技术
细胞生物学细胞生物学
材料科学材料科学材料科学

背景情况:

纳米粒子 (NP) 的细胞吸收对于应用和安全至关重要,但复杂.
颗粒大小显著影响NP细胞相互作用,较大的NP有时会增强较小的NP吸收.

研究的目的:

阐明不同尺寸的纳米粒子 (SNP) 在细胞吸收过程中相互作用的机制.
调查颗粒大小在触发内细胞分裂和促进共吸收中的作用.

主要方法:

同时暴露HeLa和A549细胞到50nm (SNP50) 和100nm (SNP100) 的纳米粒子.
传输电子显微镜 (TEM) 用于可视化个体内细胞突变事件.
使用催化酶 (CAT@SNP50) 功能化SNP50以证明增强的传递疗效.

主要成果:

确定了克拉特林介导的内细胞分裂的尺寸值:一个100nm SNP会触发它,而大约需要六个50nm SNP.
观察到100nm SNPs可以积极促进附近50nm SNPs的内细胞化和共同内化.
使用SNP100进行CAT@SNP50的增强细胞传递,与单独使用CAT@SNP50相比,导致细胞损伤更大.

结论:

揭示了一种新型的大小依赖的NP共吸收机制,由更大的NP调解,触发内细胞分裂.
这些发现影响了纳米医药的设计,这表明有意地将NP组合在一起,以有效地输送药物.
这项研究为具有尺寸分布的产品的纳米毒性评估提供了关键的见解.

关键词:

细胞吸收的时间克拉特林介导的内细胞分裂.共同暴露共同暴露的纳米粒子是什么?尺寸效应尺寸效应尺寸效应尺寸效应

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