富尔维克酸介导的高效异构化-空气性化由Paracoccus denitrificans XW11通过降低C/N比率
Yaodong Wu1, Yanan Cui1, Dongyue Li1
1National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China.
Water research
|October 4, 2024
概括
富尔维酸 (FA) 通过改善电子转移来增强异构化-有氧化脱 (HNAD),显著降低了有效去除氨的碳比要求.
科学领域:
- 环境微生物学环境微生物学
- 生物技术是生物技术.
- 污水处理 污水处理 污水处理
背景情况:
- 降低异构化-有氧脱 (HNAD) 的碳- (C/N) 比率要求对于实际的废水处理应用至关重要.
- HNAD过程对于去除污染物至关重要,但往往需要高的C/N比率,从而限制了它们的效率和成本效益.
- 探索新的策略来降低HNAD中的C/N比率需求是积极的研究领域.
研究的目的:
- 研究氧化还原媒介富尔维酸 (FA) 在降低HNAD的C/N比率要求方面的潜力.
- 为了分离和描述一个高效的HNAD细菌,Paracoccus denitrificans XW11.
- 阐明FA提高HNAD效率的基本机制.
主要方法:
- 对HNAD的Paracoccus denitrificans XW11的分离和表征.
- 全基因组测序和逆转录PCR用于识别参与化和脱的关键基因.
- 酶活性测试和转录组分析,以了解电子转移途径和基因表达.
- 在不同的C/N比率下对氨去除效率的实验性评估,含有FA和不含FA.
主要成果:
- 帕拉科库斯 (Paracoccus denitrificans) XW11显示出高的HNAD效率.
- 添加FA显著提高了氨去除效率,从71.71%提高到86.12%,C/N比为10.
- 转录组分析显示,FA促进了从碳源转移电子,通过增加NarG表达来促进化和增强脱化.
结论:
- 富尔维酸通过优化电子转移通路,有效地降低了HNAD的C/N比要求.
- 该研究确定了膜结合的缩酶 (NarG) 对于XW11中的有氧脱至关重要.
- FA提供了一种新且有前途的方法,以提高废水处理中HNAD过程的效率和适用性.
相关概念视频
Carbon-dioxide Fixation
2
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
2
Metabolism of Chemolithotrophs
3
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
3
Microbial Nutrition
3
Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
3
Inorganic Nitrogen Assimilation
6
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
6
Overview of Nitrogen Metabolism
7.9K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
7.9K
Sulfur Assimilation
1
Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...
1


