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相关概念视频

mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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pre-mRNA Processing02:01

pre-mRNA Processing

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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时间是一切:m6A何时沉积?

David Dierks1, Schraga Schwartz1

  • 1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.

Molecular cell
|October 4, 2024
PubMed
概括
此摘要是机器生成的。

N6-甲基氨酸 (m6A) 沉积主要发生在转录后. RNA的核保留时间影响m6A的积累,影响其生物发生和细胞作用.

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科学领域:

  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 在RNA生物学,RNA生物学.

背景情况:

  • N6-甲基氨酸 (m6A) 是一种普遍存在的表皮转录组修饰.
  • 确切的时间和m6A沉积的调节仍在调查中.
  • 了解m6A生物发生对于破译其功能角色至关重要.

研究的目的:

  • 为了研究m6A沉积与RNA转录相对的时间动态.
  • 确定影响m6A在RNA分子上的积累的因素.
  • 阐明这些发现对m6A生物发生和功能的影响.

主要方法:

  • 在新生和成熟RNA群体中分析m6A修饰模式.
  • 对RNA核停留时间的定量评估.
  • 核停留时间与m6A水平的相关性.

主要成果:

  • m6A沉积主要是转录后的事件.
  • RNA核停留时间的持续时间是m6A积累的关键决定因素.
  • 有证据表明,核保留和m6A固化测量之间存在直接联系.

结论:

  • m6A生物发生在很大程度上是通过转录后调节的.
  • 核停留时间作为m6A修改的关键检查点.
  • 这些见解为理解表表体转录学调节提供了一个新的框架.