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在单分子分辨率的聚变激活过程中,SARS-CoV-2 Omicron尖端剪切器的形态动态

Shuvankar Dey¹, Purba Pahari¹, Srija Mukherjee¹

¹Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur, Uttar Pradesh 208016, India.

Structure (London, England : 1993)

|October 4, 2024

概括

这种SARS-CoV-2的Omicron尖端蛋白质.

科学领域:

病毒学病毒学
结构生物学结构生物学
生物化学生物化学

背景情况:

严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 进入依赖于尖 (S) 糖蛋白介导的膜融合.
了解Omicron变种S蛋白的动态对于理解病毒进入机制至关重要.

研究的目的:

在膜融合过程中直接可视化单个SARS-CoV-2 Omicron尖端剪切器的构造变化.
阐明S2域动态,pH和离子在引发病毒进入中的作用.

主要方法:

单分子福斯特共振能量转移 (sm-FRET) 成像.
对SARS-CoV-2 Omicron伪病毒的生物化学测量.
分析尖端形形态状态的分析.

主要成果:

奥米克龙尖峰的S2域是一个动态的融合机器,可逆地在融合前和两个新的中间状态之间切换.
酸性pH值将S2转移到中间状态,促进半融合.
离子 (Ca2+) 便于从中间体过渡到融合后的状态.
低pH和Ca2+一起驱动不可逆转的S2过渡和膜融合.

结论:

SARS-CoV-2 Omicron spike S2 域表现出病毒进入所必需的动态形状变化.
pH 和 Ca2+ 起到关键触发的作用,调节 S2 构造,促进膜融合.
在S2中,合规可逆性可能会提供防止过早触发的保护.

关键词:

这就是SARS-CoV-2病毒.构造动力学构造动力学冠状病毒冠状病毒病毒膜融合融合是什么膜融合是什么单个分子成像成像技术这是一个smFRETET.病毒入口病毒入口.

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