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在实验室内研究紫外线过器对大鼠和人类甲状腺细胞的同类盐酸影响

Francesca Coperchini¹, Alessia Greco¹, Marsida Teliti²

¹Department of Internal Medicine and Therapeutics, University of Pavia, 27100, Italy.

Environmental pollution (Barking, Essex : 1987)

|October 4, 2024

概括

常见的防晒成分homosalate可能会损害甲状腺细胞. 这项研究发现,它可以降低细胞活力,改变甲状腺细胞中的基因表达,但影响因细胞类型和度而异.

科学领域:

内分泌学在内分泌学.
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

荷莫沙酸盐是防晒和个人护理产品中广泛使用的UV-B过器.
以前的研究表明,Homosalate具有雌激素和抗雄激素的特性.
有限的数据存在于Homosalate对甲状腺细胞的影响.

研究的目的:

为了在体外研究对甲状腺细胞的Homosalate暴露的潜在不良影响.
评估Homosalate对甲状腺细胞活力,增殖,基因毒性和基因表达的影响.

主要方法:

FRTL-5和正常的人类甲状腺 (NHT) 细胞在24-72小时内暴露在不同度的Homosalate中.
评估了细胞活力 (WST-1),增殖 (水晶紫) 和基因毒性 (微核染色).
测量了与甲状腺相关的基因 (TSHR,TPO,TG,NIS,PAX8) 的mRNA水平和反应性氧物种 (ROS) 生产.

主要成果:

在72小时后,同位盐降低了FRTL-5细胞在250微米的细胞活力,并在48-72小时后在2000微米的NHT细胞中降低了细胞活力.
细胞增殖没有受到homosalate暴露的影响.
同位素盐在FRTL-5细胞中的TPO和TGmRNA和NHT细胞中的TGmRNA上调,而不会改变ROS的产生.

结论:

甲状腺酸盐在体外对甲状腺细胞表现出细胞类型特异性的作用.
高度或长时间暴露于Homosalate可以诱导细胞毒性和基因毒性 (在老鼠细胞中).
甲状腺酸盐改变甲状腺基因表达 (TG和TPO),而不会影响增殖或氧化应激.

关键词:

细胞毒性细胞毒性基因毒性基因毒性荷莫萨拉特 (Homosalate) 是一种类型的盐.氧化压力是一种氧化压力.甲状腺是什么?甲状腺是什么?紫外线过器紫外线过器

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