哺乳动物卵细胞的脂质指纹的女性年龄相关的变化
Simona Bisogno1, Joanna Depciuch2,3, Hafsa Gulzar1,4
1Malopolska Centre of Biotechnology, Jagiellonian University in Kraków, Kraków, Poland.
Human reproduction (Oxford, England)
|October 4, 2024
概括
可以检测到人类卵细胞脂质谱或脂质指纹的变化,并且与母亲的年龄有关. 这些脂质变化与胚胎发育受损相关,这表明了对卵细胞质量评估的新工具.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 氧化应激 (OS) 导致早期的脂质损伤,标志着脂质滴滴 (LD) 积累,影响细胞功能.
- 我们假设受老化和OS影响的卵细胞功能可以通过LD配置文件的变化来评估,称为脂质指纹 (LF).
研究的目的:
- 为了调查卵细胞脂质谱 (LF) 是否可以通过光谱评估功能性.
- 确定LF变化,母亲年龄和卵细胞发育能力之间的关联.
主要方法:
- 来自年轻捐赠者的人类卵细胞 (n=48) 用连贯的抗斯托克斯拉曼光谱 (CARS),自发的拉曼,里埃变换红外光谱 (FTIR) 和传输电子显微镜 (TEM) 来分析.
- 用小鼠模型 (n=52野生型,n=125 Gnpat+/-) 来评估LF对胚胎发育的影响,包括外体分泌.
- 脂质染料 (BODIPY,尼罗河红色) 和流细胞计用于进一步分析.
主要成果:
- 卵细胞LF的逐渐变化,包括LD大小,面积和数量的增加,与母亲的年龄有关.
- 在个体捐赠者中可以检测到LF变异,这表明个性化卵细胞质量分级的潜力.
- 与年龄相关的LF变化涉及脂质过氧化,改变脂质组成,胡卜素积累和修改脂质双层性质.
- 在小鼠中,LF变化与胚胎外体分泌有负相关性,这可能解释了老年患者的植入失败.
结论:
- 人体卵细胞中的脂质配置变化可靠地检测,并与母亲的年龄和发育能力受损有关.
- 乳腺癌的评估为卵细胞质量评估提供了一个新的工具,并突出了卵细胞LD和胚胎外体分泌之间的功能联系.
- 了解卵细胞LF在胚胎植入中的作用,为发育生物学和生殖医学开辟了新的研究途径.
关键词:
格纳帕特格纳帕特 (Gnpat Gnpat) 是一个胡卜素是一种胡卜素.一致的反斯托克斯拉曼光谱学.外基因组是外基因组的组成部分.脂质分析 脂质分析脂质滴滴滴滴滴滴滴滴滴滴滴滴滴卵子细胞 卵子细胞更多相关视频
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