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Updated: Jun 11, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
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对3型氨酸受体的冷EM研究

Yu Seby Chen1, Maricela Garcia-Castañeda1, Maria Charalambous1

  • 1Department of Biochemistry and Molecular Biology, the Life Sciences Centre, University of British Columbia, Vancouver, BC, Canada.

Nature communications
|October 4, 2024
PubMed
概括

结构分析了瑞诺丁受体异型3 (RyR3) 离子通道. 低温电磁波显示了RyR3的存在.

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Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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